青蒿总黄酮联合有氧间歇运动训练抑制肝纤维化小鼠模型炎症反应

被引：13

作者：

成嘉祁 ^{[1
]}

龚芸芸 ^{[1
]}

谢海静 ^{[1
]}

李宏亮 ^{[2
]}

戚龙菊 ^{[3
,4
]}

机构：

[1] 南通大学医学院

[2] 南通大学杏林学院

[3] 南通市第三人民医院

[4] 南通大学护理学院

来源：

动物医学进展 | 2018年 / 39卷 / 11期

关键词：

肝纤维化; 青蒿总黄酮; 有氧间歇运动训练; 炎症反应;

D O I：

10.16437/j.cnki.1007-5038.2018.11.009

中图分类号：

R575.2 [肝硬变]; R-332 [医用实验动物学];

学科分类号：

100201 [内科学]; 100402 [劳动卫生与环境卫生学];

摘要：

利用青蒿总黄酮联合有氧间歇运动训练处理肝纤维化小鼠模型,检测肝纤维化小鼠模型中肝纤维化程度及炎症相关因子的变化,探究青蒿总黄酮及有氧间歇运动训练在治疗肝纤维化过程中的作用。取雄性Balb/c小鼠40只,刀豆蛋白A尾静脉注射成模后随机分为模型组(Model组)、黄酮组(FLA组)、运动组(TM组),联合组(FTM组)。用Masson染色检测各组小鼠肝脏纤维化程度,实时荧光定量PCR检测白细胞介素-1β(IL-1β)、IL-10、IL-6以及肿瘤坏死因子α(TNF-α)的表达;用ELISA方法检测IL-1β、IL-10以及TNF-α的表达。结果显示,与模型组相比,运动组及黄酮组肝纤维化程度显著降低,联合组纤维化程度极显著减少;黄酮组和运动组中IL-1β、IL-6、TNF-α的转录水平显著低于模型组,联合组中含量极显著降低,IL-10呈现相反的趋势;血清中IL-1β、TNF-α的含量在黄酮组和运动组中亦显著下降,联合组中含量极显著下降,而IL-10含量则相反。说明青蒿总黄酮及运动训练联合使用能够有效降低小鼠模型中肝纤维化程度,降低肝脏炎症反应,提示其能够阻滞肝纤维化向肝癌的发展。

引用

页码：52 / 57

页数：6

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