添加不同酵母培养物对瘤胃纤维分解菌群和纤维素酶活的影响

被引：39

作者：

黄庆生

王加启

不详

机构：

[1] 中国农业科学院畜牧研究所

[2] 中国农业科学院畜牧研究所北京

[3] 北京

来源：

畜牧兽医学报 | 2005年 / 02期

关键词：

16SrRNA; 定量杂交; 酵母培养物; 瘤胃细菌; 纤维分解;

D O I：

暂无

中图分类号：

S823.5 [];

学科分类号：

090502 ;

摘要：

用3种酵母培养物(YC- 1、YC 2和YC- 3)分别饲喂4头带有永久性瘤胃瘘管的肉牛,研究培养物对瘤胃发酵、纤维分解酶活性和3种纤维分解菌数量的影响,结果表明:YC 2处理的乙酸、丙酸、丁酸和总 VFA浓度显著高于对照组(P<0 .05),YC 1和YC 3处理的乙酸/丙酸比例显著降低(P<0 .01);各处理均能显著提高瘤胃内羧甲基纤维素酶、水杨苷酶和木聚糖酶的活性(P<0 .01);各处理都显著提高黄化瘤胃球菌的相对比例(P<0 .01),16SrRNA特异性寡聚核苷酸探针杂交法分析测定结果表明 3 种纤维分解菌在瘤胃细菌中所占比例为 3. 80%±0 .2%。

引用

页码：144 / 148

页数：5

共 12 条

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