Caspases在TRAIL诱导MG-63骨肉瘤细胞株凋亡中的作用

被引：3

作者：

祝少博

侯炜

喻爱喜

曹长军

方成

机构：

[1] 武汉大学中南医院显微骨科

[2] 武汉大学医学院病毒学研究所

[3] 武汉大学中南医院神经外科

[4] 武汉大学中南医院显微骨科武汉

[5] 武汉

来源：

武汉大学学报(医学版) | 2004年 / 05期

关键词：

肿瘤坏死因子相关凋亡诱导配体; 骨肉瘤; 凋亡; Caspases;

D O I：

10.14188/j.1671-8852.2004.05.004

中图分类号：

R738.1 [骨骼肿瘤];

学科分类号：

100214 ;

摘要：

目的 :初步观察并探讨人重组可溶性肿瘤坏死因子相关凋亡诱导配体 (TNF relatedapoptosis inducinglig and ,TRAIL)蛋白诱导MG 6 3骨肉瘤细胞凋亡的作用机制。方法 :用RT PCR检测TRAIL受体在MG 6 3骨肉瘤细胞上的表达 ;用MTT试验检测TRAIL对MG 6 3细胞的抑制率及加入Caspase抑制剂后TRAIL对MG 6 3骨肉瘤细胞株抑制率的变化。初步分析TRAIL诱导MG 6 3骨肉瘤细胞株凋亡的作用机理。结果 :当TRAIL单独作用于MG 6 3骨肉瘤细胞株时 ,可产生明显的抑制效果 ;而当TRAIL分别加入广谱的Caspase抑制剂zVAD FMK、Caspase 8的抑制剂zIETD FMK、Caspase 3的抑制剂zDEVD FMK与细胞株MG 6 3共同孵育时 ,TRAIL的抑制作用均被阻断。结论 :TRAIL可能是通过与细胞膜表面的死亡受体结合 ,然后激发细胞内Caspase级联反应从而诱导肿瘤细胞凋亡的。

引用

页码：497 / 500

页数：4

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