靶向人乳头瘤病毒16型-E6的siRNA对宫颈癌肿瘤生长的影响

被引：6

作者：

王晓春 ^{[1
]}

张阳德 ^{[2
]}

陈卫群 ^{[1
]}

机构：

[1] 中南大学湘雅医学院医学检验系

[2] 中南大学湘雅医院

来源：

中国现代医学杂志 | 2006年 / 24期

关键词：

宫颈癌; 裸鼠; 移植瘤; 人乳头瘤病毒16型-E6; 小分子干扰RNA;

D O I：

暂无

中图分类号：

R737.33 [子宫肿瘤];

学科分类号：

100214 ;

摘要：

目的研究靶向HPV16-E6的小分子干扰RNA抑制宫颈癌裸鼠移植瘤的生长。方法建立裸鼠宫颈癌模型,HPV16-E6siRNA瘤体内多点注射观察肿瘤的生长情况,使用HE染色方法观察肿瘤组织细胞在pSiRNA或pCON治疗后的病理学改变;免疫组化采用SP法。结果靶向HPV16-E6的小分子干扰RNA能显著抑制宫颈癌裸鼠移植瘤的生长。结论利用宫颈癌裸鼠移植瘤模型,证明靶向HPV16-E6的siRNA能够显著抑制宫颈癌裸鼠移植瘤的生长。

引用

页码：3705 / 3708

页数：4

共 12 条

[1]

RNAi:double stranded RNA directs the ATP dependent cleavage of mRNA at21￣23nucleotide intervals. ZAMORE PD,TUSCHL T,SHARP PA,et al. Cell . 2000

[2]

Bcl22constitutively suppresses p53de-pendent apoptosis in colorectal cancer cells. JIANG M,,MILNER J. Genes and Development . 2003

[3]

LE J. Uterology . 2004

[4]

Par21,a gene required for establishing polarity in C elegans embryos,encodes a putative Ser/Thr kinase that is asymmetrically distributed. GUO S,KEMPHUES KJ. Cell . 1995

[5]

The prospect of silencing disease using RNA interference. SHANKAR P,MANJUNATH N,LIEBERMAN J. The Journal of The American Medical Association . 2005

[6]

The research about HPV16,18and the HLA-I down-regulation in cervical cancer. WANG D,WANG ZD,REN BH,et al. China Journal of Chinese Materia Medica . 2004

[7]

Small in-terfering RNA and gene silencing in transgenic mice and rats. HASUWAA H,KASEDAB K,EINARSDOTTIRA T. FEBS Letters . 2002

[8]

Selective silencing of viral gene expres-sion in HPV positive human cervical carcinoma cells treated with siRNA,a primer of RNA interference. MILNER J,JIANG M. Oncegene . 2002

[9]

SiRNA agents inhibit oncogene expression and attenuate human tumor cell growth. YIN JQ,GAO J,SHAO R,et al. Journal of Experimental Therapeutics and Oncology . 2003

[10]

In vitro and in vivo growth suppression of human papilloma virus16positive cervical cancer cells by E6siRNA. YOSHINOUCHI M,,YAMADA T,KIZAKI M,et al. Molecular Therapy . 2003

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