从大豆幼胚诱导胚胎发生再生植株

被引：38

作者：

周思君

尹光初

雷勃钧

何志鸿

机构：

[1] 黑龙江省农业科学院大豆研究所

来源：

大豆科学 | 1989年 / 01期

关键词：

大豆; 组织培养; 体细胞胚胎发生; 再生植株;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

采用MS基本培养基附加高浓度的生长素(2,4—D或NAA)成功地诱导了大豆(G.max)未成熟胚的体细胞胚胎发生。2,4—D的诱导效果明显优于NAA。细胞分裂素对体细胞胚胎发生有抑制作用。适宜的维生素B1浓度为0.4ppm。体细胞胚胎发生频率随蔗糖浓度(1.5—9％)的提高而降低。体细胞胚胎经历诱导、成熟和发芽三个阶段成功地发育成完整植株。再生植株移入土壤已经获得种子。

引用

页码：39 / 45+115 +115

页数：8

共 8 条

[1] Somatic embryogenesis and plant regeneration from embryogenic callus of soybean, Glycine max L[J] . T. D. Ghazi,H. V. Cheema,M. W. Nabors.Plant Cell Reports . 1986 (6)
[2] PLANT-REGENERATION FROM CALLUS-CULTURES OF SEVERAL SOYBEAN GENOTYPES VIA EMBRYOGENESIS AND ORGANOGENESIS
BARWALE, UB
KERNS, HR
WIDHOLM, JM
[J]. PLANTA, 1986, 167 (04) : 473 - 481
[3] Plant regeneration from embryo-derived tissue cultures of soybeans[J] . J. P. Ranch,L. Oglesby,A. C. Zielinski,R. B. Horsch.In Vitro Cellular & Developmental Biology . 1985 (11)
[4] A procedure for plant regeneration from immature cotyledon tissue of soybean[J] . Paul A. Lazzeri,David F. Hildebrand,Glenn B. Collins.Plant Molecular Biology Reporter . 1985 (4)
[5] Induction of somatic embryos in cotyledonary tissue of soybean, Glycine max L. Merr[J] . Plant Cell Reports . 1984 (6)
[6] Plant regeneration from cotyledonary tissue of Glycine canescens , a perennial wild relative of soybean[J] . Jan E Grant.Plant Cell, Tissue and Organ Culture . 1984 (2)
[7] Somatic embryogenesis in cell cultures of Glycine species[J] . O. L. Gamborg,B. P. Davis,R. W. Stahlhut.Plant Cell Reports . 1983 (4)
[8] Induction and development of somatic embryos from cell suspension cultures of soybean[J] . Gregory C. Phillips,G. B. Collins.Plant Cell, Tissue and Organ Culture . 1981 (1)

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