碱蓬 c DNA表达文库的构建(英文)

被引:7
作者
马秀灵
王丽萍
张慧
机构
[1] 山东师范大学逆境植物重点实验室
[2] 山东师范大学逆境植物重点实验室 济南
[3] 济南
关键词
cDNA表达文库; 表达载体; 盐地碱蓬;
D O I
暂无
中图分类号
Q943 [植物细胞遗传学];
学科分类号
071007 ; 090102 ;
摘要
以盐生植物盐地碱蓬 (Suaeda salsa)地上部分为材料 ,提取植物总 RNA,纯化出 m R-NA,合成 c DNA第一链 ,得到双链 c DNA后 ,连接入植物表达载体 ,构建 c DNA表达文库。该文库重组子约 1 0 6。将该文库转化农杆菌 GV31 0 1 ,可直接用以转化拟南芥。
引用
收藏
页码:9 / 14
页数:6
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