中国白菜AFLP分子遗传图谱的构建

被引：36

作者：

王美

张凤兰

孟祥栋

刘秀村

赵岫云

樊治成

机构：

[1] 北京市农林科学院蔬菜研究中心

[2] 山东农业大学园艺学院

[3] 山东农业大学园艺学院北京

[4] 山东泰安

[5] 北京

来源：

华北农学报 | 2004年 / 01期

关键词：

大白菜; 双单倍体(DH); 扩增片段长度多态性(AFLP); 遗传图谱构建;

D O I：

暂无

中图分类号：

S634.1 [大白菜];

学科分类号：

090202 ;

摘要：

以白菜高抗TuMV白心株系91 112和高感TuMV桔红心株系T12 91为亲本建立的小孢子DH系作为图谱构建群体,以AFLP标记来构建白菜连锁图谱。通过对64对AFLP引物组合的筛选,利用20对引物得到了263个AFLP多态性位点。经Mapmaker/EXP3.0软件处理,构建了1张含255个标记位点,10个连锁群,覆盖长度为883.7cM的连锁图。255个AFLP位点中偏离孟德尔遗传的比率(P<0.05)为15 0%和27 5%(P<0.01)。

引用

页码：28 / 33

页数：6

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