心室肌层的副交感神经支配——HRP-AChE结合法的研究

被引:5
作者
王健本
李光千
祝善乐
殷树仪
张祜曾
机构
[1] 同济医科大学解剖学教研室
关键词
副交感神经; 胆碱能神经元; 心脏器官内神经; HRP; AchE; 心脏;
D O I
10.16098/j.issn.0529-1356.1987.03.008
中图分类号
学科分类号
摘要
于11只家兔左、右心室前壁肌层内,注射20~50%HRP生理盐水溶液10~20μl。取心房后壁、窦房结区和房室结区,冰冻或恒冷箱切片,厚25~40μm,以HRP(TMB法)-AChE结合法进行孵育。心房后壁心外膜层内,少数AChE强阳性神经节细胞胞质内含HRP与TMB反应所生成的蓝色颗粒,说明这种HRP-AChE双标细胞的突起,分布于心室前壁肌层。一般认为,AChE强阳性神经细胞是胆碱能神经元。本研究进一步证实,心室肌层有副交感神经支配,支配左、右心室前壁的副交感节后神经元,主要位于心房后壁心外膜层内。
引用
收藏
页码:246 / 250+339 +339
页数:6
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