纳米金修饰电极和探针载体的DNA电化学发光分析方法研究

被引：4

作者：

王辉

李延

漆红兰

张成孝

机构：

[1] 陕西师范大学化学与材料科学学院

来源：

陕西师范大学学报(自然科学版) | 2006年 / 04期

关键词：

核酸分析; 金纳米粒子; 电化学发光;

D O I：

10.15983/j.cnki.jsnu.2006.04.018

中图分类号：

Q78 [基因工程（遗传工程）];

学科分类号：

071007 ; 0836 ; 090102 ;

摘要：

提出以纳米金修饰电极和以纳米金粒子作DNA探针载体的电化学发光检测DNA新方法.首先将纳米金自组装在金电极上,再将含巯基的目标ss-DNA固定于纳米金修饰的电极上,然后与以纳米金粒子作载体的电化学发光DNA探针进行杂交反应,将此电极做工作电极,在含有三丙胺的溶液中进行电化学发光测量.在选定实验条件下,检测囊肿纤维DNA片断(20 base)的线性范围为1.0×10-12～1.0×10-9mol/L,相关系数为0.9954,检出限为5.0×10-13mol/L.实验结果表明,纳米金具有较大的比表面积,可增强DNA在电极上的固定量,从而增强电化学发光检测信号,提高方法的灵敏度.

引用

页码：69 / 72

页数：4

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