脂肪干细胞向软骨细胞方向诱导的初步研究

被引：28

作者：

余方圆

卢世璧

袁玫

黄靖香

赵斌

孙明学

机构：

[1] 解放军总医院骨科研究所

[2] 解放军总医院骨科研究所北京

[3] 北京

来源：

中国矫形外科杂志 | 2004年 / 10期

关键词：

干细胞; 脂肪干细胞; 骨髓干细胞:; 生长因子; 诱导分化;

D O I：

暂无

中图分类号：

R329.2 [人体细胞学];

学科分类号：

100101 ;

摘要：

目的 :研究脂肪干细胞的分离及向软骨方向定向诱导的方法。方法 :从成年兔颈后部或腹股沟处脂肪组织取材 ,酶消化法分离、培养脂肪干细胞 ;免疫荧光测定CD3 4 抗原的表达 ;流式细胞仪测定CD3 4 抗原百分比 ;分别用15 %牛血清、少血清 (5 % )及无血清诱导培养基定向软骨细胞方向诱导 ;组化及免疫组化鉴定软骨细胞特性。结果 :从兔脂肪组织中能分离出生长旺盛的干细胞 ,CD3 4 抗原表达阳性 ,表达百分比与骨髓干细胞无明显差异 ;定向诱导后表现出软骨细胞的特性 ,诱导效果无血清诱导培养基效果最好。结论 :从兔脂肪组织中能分离出干细胞 ;生物学特性与骨髓干细胞相似 ;能向软骨细胞方向诱导 ;无血清诱导培养基效果最好。

引用

页码：42 / 44

页数：3

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