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Enhancing cellular immune response to HBV M DNA vaccine in mice by codelivery of interleukin-18 recombinant
被引：21
作者：
陈建忠
朱海红
刘克洲
陈智
机构：
[1] InstituteofImmunology,SchoolofMedicine,ZhejiangUniversityHangzhou,China,InstituteofInfectiousDiseases,FirstAffiliatedHospital,SchoolofMedicine,ZhejiangUniversityHangzhou,China,InstituteofInfectiousDiseases,FirstAffiliatedHospital,SchoolofMedicine,ZhejiangU
关键词：
D O I：
暂无
中图分类号：
R392 [医学免疫学];
学科分类号：
100108 [医学免疫学];
摘要：
Objective: To investigate the effect of interleukin-18 (IL-18) on immune response induced by plasmid encoding hepatitis B virus middle protein antigen and to explore new strategies for prophylactic and therapeutic HBV DNA vaccines. Methods: BALB/c mice were immunized with pCMV-M alone or co-immunized with pcDNA3-18 and pCMV-M and then their sera were collected for analysing anti-HBsAg antibody by ELISA; splenocytes were isolated for detecting specific CTL response and cytokine assay in vitro. Results: The anti-HBs antibody level of mice co-immunized with pcDNA3-18 and pCMV-M was slightly higher than that of mice immunized with pCMV-M alone, but there was not significantly different (P>0.05). Compared with mice injected with pCMV-M, the specific CTL cytotoxity activity of mice immunized with pcDNA3-18 and pCMV-M was significantly enhanced (P<0.05) and the level of IFN- in supernatant of splenocytes cul-tured with HBsAg in vitro was significantly elevated (P<0.05) while the level of IL-4 had no significant difference (P>0.05). Conclusion: The plasmid encoding IL-18 together with HBV M gene DNA vaccines may enhance specific TH1 cells and CTL cellular immune response induced in mice, so that IL-18 is a promising immune adjuvant.
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页数：5
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