渗透胁迫对玉米幼苗叶片不同形态多胺含量的影响(英文)

被引:9
作者
刘怀攀 [1 ]
纪秀娥 [2 ]
刘天学 [1 ]
史留功 [2 ]
李潮海 [1 ]
机构
[1] 河南农业大学河南省作物生长发育调控重点实验室
[2] 周口师范学院植物学重点实验室
关键词
玉米; 多胺形态; 渗透胁迫;
D O I
暂无
中图分类号
S513 [玉米(玉蜀黍)];
学科分类号
0901 ;
摘要
用高压液相色谱法,研究了玉米品种农大108(抗旱性较强)和掖单13(抗旱性较弱)幼苗叶片中游离态(free,f)、酸可溶性共价结合态(AS-conjugated)和酸不溶性共价结合态(AIS-bound)多胺(polyamine,PA)与渗透胁迫的关系。结果发现,渗透胁迫7 d,农大108的fSpd和fSpm的含量显著(P<0.05)上升,而掖单13的fPut的升幅显著(P<0.05)。用S-腺苷蛋氨酸脱羧酶(SAMDC)抑制剂甲基乙二醛-双(鸟嘌呤腙)(MGBG)处理农大108,显著抑制了渗透胁迫诱导的fSpd和fSpm的增加,并且加重了渗透胁迫伤害,用外源Spd处理掖单13,则显著促进了渗透胁迫诱导的fSpd和fSpm的增加,并且减缓了渗透胁迫的伤害。渗透胁迫下,农大108幼苗叶片中AS-conjugated-PA和AIS-bound-PA的上升幅度都显著大于掖单13。用转谷氨酰胺酶(TGase)抑制剂菲咯啉(o-Phen)处理,则抑制了渗透胁迫诱导的AIS-bound-PA含量的上升,并加重了渗透胁迫的伤害。上述结果说明,玉米幼苗叶片中的fSpdf、Spm、AS-conjugated-PA和AIS-bound-PA的升高有利于增强幼苗的抗渗透性。
引用
收藏
页码:1430 / 1436
页数:7
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