我国霍乱弧菌的脂肪酸分型研究

被引:10
作者
王瑞白 [1 ]
刘海洪 [2 ]
邱海燕 [1 ]
宋亚军 [2 ]
杨瑞馥 [2 ]
阚飙 [1 ]
机构
[1] 中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室
[2] 军事医学科学院微生物流行病学研究所病原微生物生物安全国家重点实验室
关键词
霍乱弧菌; 脂肪酸; 分型;
D O I
10.13523/j.cb.20070604
中图分类号
R378.3 [弧菌];
学科分类号
100103 ; 100705 ;
摘要
目的:对脂肪酸分型方法在霍乱弧菌菌株鉴定、菌株相似性分析等方面的应用价值进行评价。方法:选取了分离自我国的两个主要致病血清群的194株霍乱弧菌菌株(1961年以来的ElTor型和1992年以来的O139群),提取脂肪酸,应用MIDI公司的脂肪酸分型系统,进行数据分析。结果:检测的所有菌株都含有的脂肪酸成分有13种。霍乱弧菌的判断符合率为88.6%。二维聚类分析没有成明显可区分的群,O139群霍乱弧菌的脂肪酸组成与O1群的相似,产毒与非产毒霍乱弧菌的脂肪酸成分没有显著差异。结论脂肪酸分型对弧菌种的快速鉴定有应用价值,对霍乱弧菌的现场分离鉴定有辅助意义,在小样本暴发资料的研究中能够反映菌株之间的亲缘关系,但其对霍乱弧菌种属内的各种特征性菌群不具有鉴别能力。
引用
收藏
页码:15 / 21
页数:7
相关论文
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