同型半胱氨酸对血管内皮细胞血凝素样氧化型低密度脂蛋白受体1 mRNA表达及氧化型低密度脂蛋白摄取的影响

被引:9
作者
李全忠
朱雯霞
赵水平
机构
[1] 中南大学湘雅第二医院心内科
[2] 南华大学心血管病研究所
[3] 中南大学湘雅第二医院心内科 湖南省长沙市
[4] 湖南省衡阳市
[5] 湖南省长沙市
关键词
同型半胱氨酸; 内皮细胞; 血凝素; 受体; 脂蛋白,低密度;
D O I
暂无
中图分类号
R543.5 [动脉疾病];
学科分类号
1002 ; 100201 ;
摘要
为了研究同型半胱氨酸对血管内皮细胞血凝素样氧化型低密度脂蛋白受体 1mRNA表达及对氧化型低密度脂蛋白摄取的影响 ,分别在培养的人脐静脉内皮细胞中加入不同浓度的同型半胱氨酸孵育 48h后 ,利用逆转录—聚合酶链反应检测血凝素样氧化型低密度脂蛋白受体 1mRNA表达的改变 ;采用放射配基法检测细胞对氧化型低密度脂蛋白的摄取。结果发现 ,随着同型半胱氨酸浓度的增加 ,血凝素样氧化型低密度脂蛋白受体 1mRNA表达增加 ;同时对氧化型低密度脂蛋白的摄取率也增加。提示同型半胱氨酸能促进脐静脉血管内皮细胞血凝素样氧化型低密度脂蛋白受体 1mRNA的表达和对氧化型低密度脂蛋白的摄取 ,此效应可能与同型半胱氨酸致动脉粥样硬化作用有关
引用
收藏
页码:324 / 326
页数:3
相关论文
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