水分条件对水稻土微生物群落多样性及活性的影响

被引:43
作者
刘岳燕
姚槐应
黄昌勇
机构
[1] 浙江大学环境与资源学院
关键词
水稻土; BIOLOG碳素利用; 磷脂脂肪酸(PLFA); 基础呼吸; 酶活性;
D O I
暂无
中图分类号
S511 [稻];
学科分类号
摘要
采用BIOLOG碳素利用法、磷脂脂肪酸(PLFA)法和土壤酶活性测定等方法比较了三种水分条件(淹育、淹育晾干、非淹育)对水稻土微生物群落多样性及活性的影响。结果表明,淹育处理水稻土的脱氢酶、蔗糖酶活性明显高于淹育晾干和非淹育处理,并导致该土壤的基础呼吸升高。BIOLOG碳素利用法表明,非淹育处理的微生物群落平均吸光值(AWCD)显著低于淹育和淹育晾干处理。磷脂脂肪酸(PLFA)实验发现,淹育水稻土的真菌特征脂肪酸(18∶2w6,9c)所占比例减少,真菌特征脂肪酸(18∶2w6,9c)与细菌特征脂肪酸(15∶0i+15∶0a+16∶0i+16∶1w5c+17∶0i+17∶0a+17∶0cy+17∶0+18∶1w7c+19∶0cy)的比值下降;水分条件变化没有改变土壤微生物环丙基脂肪酸19∶0cy的相对丰度,但非淹育处理的环丙基脂肪酸17∶0cy相对丰度明显高于另外二种处理。BIOLOG碳素利用法的群落水平生理剖面(CLPP)和磷脂脂肪酸(PLFA)测定结果经聚类分析后,发现淹育和淹育晾干处理的土壤微生物多样性在较低的距离尺度可聚成一类,且与非淹育土壤具有明显差异。淹育水稻土与淹育晾干相比,尽管土壤微生物群落结构和功能多样性有一定的相似性,但微生物的种群组成和活性仍发生了较大的变化。
引用
收藏
页码:828 / 834
页数:7
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