人参皂甙Rg1对自由活动大鼠突触可塑性的影响及其作用机制＜英文>

被引：14

作者：

王晓英

张均田

机构：

[1] 中国 100050

[2] 中国医学科学院中国协和医科大学药物研究所药理研究室

[3] 中国医学科学院中国协和医科大学药物研究所药理研究室北京

来源：

Acta Pharmacologica Sinica | 2001年 / 07期

关键词：

人参; 皂甙类; 长时程增强; 突触; 齿状回; GAP-43蛋白质;

D O I：

暂无

中图分类号：

R285 [中药药理学];

学科分类号：

1008 ;

摘要：

目的:研究人参皂甙Rg1对自由活动大鼠突触功能可塑性的影响及作用机制。方法:应用细胞外微电极记录技术,大鼠埋植电极后d6予以Rg1(10,30mg/kg,ip)12d,记录(直至停药后d3)其齿状回群体峰电位(PS)。大鼠给予Rg1(10,30mg/kg,ip)12d,据Timm染色法观察海马CA3区苔辞纤维出芽情况。以免疫组化技术检测齿状回颗粒细胞层GAP-43表达水平。结果:1)Rg1可显著降低诱发PS的阈值,提高清醒自由活动大鼠的突触传递效能,诱导LTP形成,停药3d后,LTP仍可维持。2)Rg1组大鼠齿状回颗粒细胞层及齿状回门区GAP-43表达显著增加。3)Timm染色显示海马CA3区苔藓纤维出芽增加。结论:Rg1可使自由活动大鼠PP-DG突触传递效能发生以LTP为主的可塑性变化,其机制为齿状回颗粒细胞GAP-43表达增加,其投射靶区海马CA3苔藓纤维明显出芽增加,这呈正反馈性增强了突触传递效能。

引用

页码：83 / 88

页数：6

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