单细胞凝胶电泳在遗传学损伤检测中的应用

被引:5
作者
柳鑫
机构
[1] 解放军第医院全军优生优育中心 沈阳
关键词
彗星试验; 人类生物监测; 职业暴露; 环境污染; 生活方式;
D O I
10.13404/j.cnki.cjbhh.2003.03.004
中图分类号
R394 [医学遗传学];
学科分类号
0710 ; 071007 ;
摘要
单细胞凝胶电泳或彗星试验是在暴露人群中评价遗传学损伤的新式方法。这种分析可以快速的探测单一细胞中不同形式的DNA损伤 ,因此适于在暴露人群分析应用。本文将对彗星试验在临床研究及监测职业、环境、合理饮食、生活方式等对DNA损伤的危险性 ,以及在DNA损伤与修复研究中的应用等方面进行综述。
引用
收藏
页码:9 / 10
页数:2
相关论文
共 18 条
[1]  
Interactingeffectsofnutritionandsocialclassdifferentialsonfertilityandinfantmortalityinthepre-industrialpopulation. ScottS,DuncanCJ. Populstud . 2000
[2]  
ProtectionagainstultravioletB-andC -inducedDNAdamageandskincarcinogenesisbytheflowersofPrunuspersicaextract. HeoMY,KimSH,YangHEetal. Mutation Research . 2001
[3]  
DNA integrity in human spermatozoa: relationships with semen quality. Irvine D S,Twigg J P,Gordon E L,Fulton N,Milne P A,Aitken R J. Journal of Andrology . 2000
[4]  
Application of integrated genetic monitoring: the optimal approach for detecting environmental carcinogens. M S Legator, W W Au. Journal of Environmental Health . 1994
[5]  
Singlecellgelelectrophoresisassayanewtechniqueforhumanbiomonitoringstudies. FekaduKassie,WolframParzefall,SiegfriedKnasmuller. Mutation Research . 2000
[6]  
Whyatt.Biomakers and molecular epidemiology in mutation/cancer research. F P Pertera. R. M. Mutation Research . 1994
[7]  
Microgel electrophresis assay(comet test) and SCE analysis in human lymphocytes from 100 normal subjects. C Betti, T Davini, L Giannessi, et al. Mutation Research . 1994
[8]  
EvaluationofthecapacityoftheSCGEassaytoassessthegenotoxicityofbioma terials. Chauvel-LebreDJ,AuroyP,Tricot-DoleuxSetal. Biomaterials . 2001
[9]  
Exercise-inducedDNAeffectsinhumanleukocytesarenotaccompaniedbyincreasedfor mationof8-hydroxy-2-deoxyguanosineorinductionofmi cronuclei. A .Hartmann,S .Pfuhler,C .Dennogetal. FreeRadicalBiolMed . 1998
[10]  
Chromosomal aberrations and alkaline comet assay in families with habitual abortion. V. Baltaci, N. Aygun, D. Akyol et al. Mutation Research . 1998