PCR-RFLP 方法测定 ras 癌基因点突变

被引:4
作者
王俊茹
邓国仁
刘为纹
吕有勇
李文梅
机构
[1] 第三军医大学第一附属医院消化科
[2] 北京市肿瘤防治研究所
[3] 北京市肿瘤防治研究所 重庆
[4] 北京
[5] 重庆
关键词
ras 基因; 点突变; PCR; RFLP;
D O I
暂无
中图分类号
学科分类号
摘要
曾使用 PCR-RFLP 方法分析过 c-Ha-ras 癌基因第12密码子的点突变.因N-ras 基因第12位密码子、K-ras 基因第12和13位密码子无已知的限制性内切酶的酶切位点,不能使用 PCR-RFLP 方法分析这些位点的突变.在 PCR 引物的3′端引入一个误配的碱基使之正好成为某限制性内切酶的酶切位点,这样便能使用PCR-RFLP 技术分析 c-Ha-ras 基因第61位、N-ras 基因第12位、K-ras 基因第12和13位密码子的点突变.
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页码:146 / 149
页数:4
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