无机砷对人支气管上皮细胞p16基因甲基化及表达的影响

被引:15
作者
卢光明
蔡庆
张文
乌正赉
戚其平
姚孝元
丛涛
机构
[1] 解放军总医院基础医学研究所,空军总医院分子生物学中心,空军总医院分子生物学中心,中国协和医科大学流行病教研室,中国预防医学科学院环境卫生监测所,中国预防医学科学院环境卫生监测所,解放军总医院基础医学研究所北京,北京
关键词
砷剂; 基因,p16; DNA甲基化; 基因表达;
D O I
暂无
中图分类号
R329.2 [人体细胞学];
学科分类号
100101 ;
摘要
目的 观察亚砷酸钠 (NaAsO2 )和砷酸钠 (Na3 AsO4 )对人支气管上皮BEP2D细胞p16基因甲基化及表达的影响。方法 采用甲基化特异性PCR(MSP)及RT PCR等技术观察分析BEP2D细胞p16基因甲基化和表达水平。结果  (1)NaAsO2 (0 0 16~ 2 μmol/L)和高浓度组Na3 AsO4 (80和16 0 μmol/L) 具有促进BEP2D细胞p16基因二核苷酸CpG岛甲基化作用 ;而低浓度组Na3 AsO4 (2 0和40 μmol/L) 不能使BEP2D细胞p16基因CpG岛发生甲基化。 (2 )无机砷可使BEP2D细胞p16基因表达下降 ,且NaAsO2 较Na3 AsO4 更为明显。结论 无机砷可引起BEP2D细胞p16基因CpG岛甲基化程度增高和表达水平下降 ,提示p16基因高甲基化是无机砷致癌的可能途径之一。
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页码:25 / 28
页数:4
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