突触后密度蛋白93基因敲除抑制血小板活化因子性神经元损害

被引：1

作者：

徐运

黄嵘

管得宁

王岚

王中原

王晓云

陶元祥

机构：

[1] 南京大学医学院附属鼓楼医院神经内科南京大学医药生物技术国家重点实验室

[2] 美国霍普金斯大学

来源：

临床神经病学杂志 | 2004年 / 06期

关键词：

血小板活化因子; PSD93基因敲除; 神经元; 细胞凋亡;

D O I：

暂无

中图分类号：

R741 [神经病学];

学科分类号：

摘要：

目的　探讨血小板活化因子 (PAF)所致的神经元毒性损伤是否受突触后密度蛋白 93(PSD93 )基因调控。方法　用PAF处理培养的野生型和PSD93 基因敲除型的小鼠皮质神经元 ;经碘化物 (PI) /钙黄绿素(Calcein)染色 ,荧光显微镜观察并计算细胞死亡率 (% ) ;细胞生存能力检测 (MTT)神经元生存能力 ;免疫印迹法测定PSD93 和PSD95蛋白的表达。结果　野生型神经元表达PSD93 和PSD95蛋白 ,PSD93 基因敲除型只表达PSD95,不表达PSD93 ;基因敲除型的小鼠皮质神经元凋亡明显低于野生型 (P <0 0 5 ) ,生存能力高于野生型(P <0 0 1)。结论　PSD93 基因敲除抑制PAF所致的神经元损害

引用

页码：424 / 426+481 +481

页数：4

共 8 条

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