用流式细胞仪分析抗生素后效应中细菌的生物学特征

被引：3

作者：

聂大平

李振国

马荣

丁侠

常青燕

机构：

[1] 大连医科大学附属第二医院

[2] 大连医科大学检测系

[3] 大连市第六人民医院

来源：

中国抗生素杂志 | 2002年 / 10期

关键词：

流式细胞仪; 抗生素后效应; 大肠埃希氏菌; 铜绿假单胞菌;

D O I：

10.13461/j.cnki.cja.002644

中图分类号：

R915 [药物生物学];

学科分类号：

摘要：

目的　了解亚胺培南、环丙沙星、庆大霉素、头孢他啶四种抗生素对大肠埃希氏菌、铜绿假单胞菌抗生素后效应 (PAE)中细菌的大小及核酸含量的改变。方法　将处于亚胺培南、环丙沙星、庆大霉素、头孢他啶后效应中的大肠埃希氏菌、铜绿假单胞菌 ,用碘化丙啶 (PI)染色 ,流式细胞仪检测。结果　亚胺培南、环丙沙星PAE中 ,两种菌体积与核酸含量均增大 ;庆大霉素PAE中 ,两种菌体积与核酸含量不变 ;头孢他啶作用后的大肠埃希氏菌体积、核酸含量增大 ,铜绿假单胞菌没有变化。结论　这些变化与抗生素作用机制有关。用流式细胞仪能快速、直观分析细菌的生物学特征。

引用

页码：613 / 616

页数：4

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