米曲霉RIBl28耐酸性木聚糖酶的研究

被引：10

作者：

陆健

曹钰

陈坚

若林三郎

机构：

[1] 江南大学生物工程学院

[2] 日本国税厅酿造研究所酶工程研究室

来源：

无锡轻工大学学报 | 2001年 / 06期

关键词：

木聚糖酶; 米曲霉; 筛选; 纯化; 性质;

D O I：

暂无

中图分类号：

O629 [天然化合物];

学科分类号：

摘要：

通过RBB—Xylan筛选平板和固体培养方法获得了产木聚糖酶菌株米曲霉 (Aspergillusoryzae)RIBl2 8.在液体发酵时木聚糖是有效的诱导底物 .通过离子交换和凝胶过滤色谱纯化得到了一个耐酸性木聚糖酶 (木聚糖酶B) .它的相对分子质量为 6 5 0 0 0 ,最适作用温度和 pH分别为 5 5℃ (pH 6 .5 ) ,它在 5 0℃时很稳定 ,并且在 pH 2 .0时仍保留相当高的活性 .木聚糖酶B含有较多的天冬氨酸、丝氨酸、苏氨酸和丙氨酸 .

引用

页码：608 / 611

页数：4

共 9 条

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