猪苓多糖对小鼠腹腔巨噬细胞一氧化氮生成、iNOS活性和细胞内还原型谷胱甘肽含量的影响

被引：12

作者：

陈伟珠

侯敢

张海涛

机构：

[1] 广东医学院生物化学教研室,广东医学院生物化学教研室,广东医学院生物化学教研室广东湛江,广东湛江,广东湛江

来源：

广东医学院学报 | 2003年 / 04期

关键词：

猪苓多糖; 一氧化氮; 诱导型一氧化氮合酶; 脱甘肽; 巨噬细胞;

D O I：

暂无

中图分类号：

R285 [中药药理学];

学科分类号：

摘要：

目的 :观察猪苓多糖 (PPS)对小鼠腹腔巨噬细胞一氧化氮 (NO)生成及其免疫调节作用和抗肿瘤作用机制。方法 :采用Griess反应、荧光法和DTNB比色法分别测定不同剂量的PPS作用小鼠腹腔巨噬细胞的NO生成量、诱导型一氧化氮合酶 (iNOS)活性及细胞内还原性谷胱甘肽 (GSH)含量的变化。结果 :PPS能使小鼠腹腔巨噬细胞NO生成增加 ,i NOS活性增高 ,并呈作用剂量依赖关系 ;PPS在刺激小鼠腹腔巨噬细胞NO合成同时 ,伴有细胞内GSH水平的降低 ,呈负相关 (P <0 .0 5 )。结论 :PPS能提高小鼠腹腔巨噬细胞iNOS活性 ,增加NO合成 ,消耗细胞内的GSH。提示细胞内GSH可能起到调节巨噬细胞NO生成和保护宿主细胞免受NO介导的细胞毒作用

引用

页码：319 / 320+323 +323

页数：3

共 10 条

[1]

Chanq Cl,Liao JC,Kuo L.Macrophage arginase promotes tumor cell growth and suppresses nitric oxide-mediated tumor cytotoxicity. Cancer Research . 2001

[2]

Coren KD.Evidence for antiviral effect of nitric oxide: inhibition of herpes simplex virus type-1 replication. The Journal of Clinical Investigation . 1993

[3]

Ghigo D,Alessio P,Foco A,et al.Nitric oxide synthesis is impaired in glutathione-depleted human umbilical vein endothelial cells. American Journal of Physiology . 1993

[4]

Barnes PJ,Liew FY.NO and asthmatic inflammation. Immunology Today . 1995

[5]

Liu WK.Activation of peritoneal macrophages by polysaccharopeptide from the mushroom, coriolus versicolor. Immunopharmacology . 1993

[6]

Li Y,Ito N,Suzuki T,et al.Dexamathasone inhibits nitric oxide-mediated cytotoxicity via effects on both macrophages and target cells. Immunopharmacology . 1995

[7]

Wang W,Inoue N,Nakayama T,et al.An assay method for nitric oxide synthase in crude samples by determining product NADP. Analytical Biochemistry . 1995

[8]

Ding AH,Nathan CF,Stuehr DJ.Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages: comparison of activating cytokines and evidence for independent production. J Immunol . 1988

[9]

Lorsbach RB,Russell SW.A specific sequence of stimulation is required to induce synthesis of the antimicrobial molecule nitric oxide by mouse macrophages. Infection and Immunity . 1992

[10]

Soini Y,Kalhos K,Puhakka A,et al.Expression of inducible nitric oxide synthase in healthy pleura and in malignant mesothelioma. British Journal of Cancer . 2000

← 1 →