胰岛素样生长因子-1对血管平滑肌细胞PI3K/PTEN信号通路的影响

被引：2

作者：

吴兴利

杨丁友

杨中苏

王士雯

赵玉生

机构：

[1] 解放军总医院老年心血管病研究所,解放军总医院老年心血管病研究所,解放军总医院老年心血管病研究所,解放军总医院老年心血管病研究所,解放军总医院老年心血管病研究所北京,北京,北京,北京,北京

来源：

中国应用生理学杂志 | 2004年 / 03期

关键词：

磷脂酰肌醇-3激酶; PTEN; 胰岛素样生长因子-1; 血管平滑肌细胞;

D O I：

10.13459/j.cnki.cjap.2004.03.019

中图分类号：

R341 [];

学科分类号：

1001 ;

摘要：

目的 :探讨胰岛素样生长因子 1(IGF 1)促血管平滑肌细胞 (VSMC)增殖的细胞内信号转导机制。方法 :体外培养的兔血管平滑肌细胞分 3组处理 ,以细胞计数、噻唑盐比色法测定细胞增殖能力 ,以磷脂酰肌醇 3激酶 (PI3 K)特异性抑制剂渥漫青霉素 (WT)孵育细胞间接反映PI3 K作用。WesternBlot定量磷酸酶PTEN表达水平 ,免疫沉淀、特异底物diC16PIP3 绿色试剂法测定PTEN脂质磷酸酶活性。结果 :IGF 1(10 0 μg/L)使细胞计数及MTT比色A值分别增加至对照组的 2 .8倍和 3.8倍 ,WT抑制VSMC增殖 ,并完全逆转IGF 1的作用 (均P <0 .0 1)。各浓度IGF 1对PTEN蛋白表达水平无明显影响 ,其对PTEN活性的抑制呈浓度 (10～ 10 0 μg/L)及时间 (3min～ 2 4h)依赖性 (均P <0 .0 1)。结论 :IGF 1促VSMC增殖作用与活化PI3 K蛋白激酶的促生长活性及抑制PTEN脂质磷酸酶的负性调节细胞生长作用有关

引用

页码：52 / 55

页数：4

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