不同培养时间软骨细胞的生物学特性

被引：6

作者：

林建华

吴朝阳

许卫红

机构：

[1] 福建医科大学附属第一医院骨科!福州

来源：

福建医科大学学报 | 2000年 / 02期

关键词：

组织学技术; 软骨; 细胞,培养的; 兔;

D O I：

暂无

中图分类号：

R329-33 [组织学技术];

学科分类号：

摘要：

目的　探讨软骨细胞在体外不同培养时间的生物学特性。　方法　把软骨细胞置盖玻片上培养 ,观察不同培养时间细胞的形态学变化和增殖能力 ;应用阿新蓝和三色染色 ,观察细胞在培养过程中酸性粘多糖 (GAG)和胶原分泌情况。　结果　软骨细胞经体外单层培养 ,传代 4～ 5代后 ,细胞形态逐渐由多角形变为梭形 ,基质分泌减少 ,GAG和胶原染色变浅。　结论　软骨细胞在体外培养 3周左右是作为软骨组织工程的最佳种子细胞

引用

页码：123 / 125

页数：3

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