蛋白聚糖Versican V1区诱导小鼠脊柱炎和骶髂关节炎模型的建立

被引：1

作者：

朱剑

黄烽

赵绵松

张莉芸

王炎焱

张一平

机构：

[1] 解放军总医院风湿科

[2] 美国加利福尼亚大学Irvine分校北京

[3] 北京

[4] CA

来源：

解放军医学杂志 | 2004年 / 06期

关键词：

脊柱炎,强直性; 疾病模型,动物; 自身免疫; 蛋白聚糖类,Versican;

D O I：

暂无

中图分类号：

R593.23 [强直性脊柱炎];

学科分类号：

1002 ; 100201 ;

摘要：

目的　建立自身免疫性强直性脊柱炎(AS)实验动物模型 ,并探讨重组人蛋白聚糖Versican的G1区多肽 (VG1 )在其脊柱和骶髂关节炎发病中的作用。方法　用自然折叠的VG1和完全弗氏佐剂免疫BALB/c小鼠 ,诱导产生脊柱炎和骶髂关节炎 ,建立自身免疫性AS实验动物模型。采用组织病理学方法显示脊柱炎小鼠的发病程度和病理学特征。结果　用VG1和完全弗氏佐剂免疫的实验组小鼠脊柱炎和骶髂关节炎的发病率分别为 35 0 %和 1 2 5 % ,未出现外周炎的临床症状。组织病理学结果显示 ,脊柱韧带与椎间盘连接处、椎间盘和骶髂关节有大量的单个核细胞浸润。结论　成功建立了VG1诱导的小鼠脊柱炎模型 ,并发现针对蛋白聚糖Versican的免疫反应参与了BALB/c小鼠脊柱炎和骶髂关节炎的发病 ,为进一步深入研究人类AS的发病机制及其临床治疗提供了有价值的实验材料

引用

页码：486 / 488

页数：3

共 9 条

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