离线2D-LC-MS系统的建立及其用于人肝蛋白质组学的研究

被引：3

作者：

王静

许雪姣

于雁灵

刘银坤

杨原

机构：

[1] 复旦大学化学系,复旦大学化学系,复旦大学化学系,复旦大学蛋白质组研究中心,复旦大学化学系上海,复旦大学蛋白质组研究中心,上海,上海,复旦大学蛋白质组研究中心,上海,上海,复旦大学蛋白质组研究中心,上海,上海,复旦大学肝癌研究所,上海,上海,复旦大学蛋白质组研究中心,上海

来源：

高等学校化学学报 | 2004年 / 12期

关键词：

多维色谱-质谱联用仪; 离线2D-LC-MS; 水溶性蛋白;

D O I：

暂无

中图分类号：

O657.63 [质谱分析];

学科分类号：

070302 [分析化学];

摘要：

A lab-made off line 2D-LC-MS system was developed for protein analysis. In case the off line 2D-LC was utilized for peptide separation, and the working conditions for strong cation exchange(SCX) chromatography and the succeeding reversed phase(RP) chromatography can be individually optimized, contrasted to the on-line system. The experimental results show that the mass fractions of acetonitrile for the SCX separation can be increased form 5% as references reported up to 20% so that the separation of peptides has been substantially improved. The off line system has been applied to the analysis of proteins extracted from the cancer tissue of nude mice inoculated by a human-hepatocellular-carcinoma cell-line D20. Some extreme basic proteins(pI>10) and larger proteins(M w>200 000), which are difficult to be isolated by the traditional 2D-PAGE method, have been identified in D20 sample.

引用

页码：2247 / 2249

页数：3