用MALDI-TOF MS技术快速鉴定临床分离棒状杆菌属细菌

被引：9

作者：

徐志江

周宏伟

孙谦

胡燕燕

陈功祥

张嵘

机构：

[1] 浙江大学医学院附属第二医院检验科

来源：

临床检验杂志 | 2012年 / 30卷 / 09期

关键词：

棒状杆菌属; 质谱; 16S rRNA;

D O I：

10.13602/j.cnki.jcls.2012.09.003

中图分类号：

R446.5 [微生物学检验];

学科分类号：

100208 ;

摘要：

目的评价基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF MS)快速鉴定临床分离的非白喉棒状杆菌的价值。方法收集本院58株非白喉棒状杆菌,用MALDI-TOF MS和16S rRNA基因测序两种方法进行鉴定和比较。用MALDI-Biotyper软件构建不同棒状杆菌MALDI-TOF MS蛋白系统发育树。结果 58株非白喉棒状杆菌中,54株MALDI-TOF MS与16S rRNA基因测序鉴定结果一致,包括34株纹带棒状杆菌(Corynebacterium straitum)、11株杰氏棒状杆菌(C.jeikeium)、3株C.resistens、2株解葡萄糖苷棒状杆菌(C.glucuronolyticum)、2株黏金色棒状杆菌(C.aurimucosum)和2株无枝菌酸棒状杆菌(C.amycolatum)。4株16S rRNA基因测序无法鉴定到种的菌株中,MALDI-TOF MS鉴定为2株产黏棒状杆菌(C.mucifaciens)、1株C.singulare和1株假白喉棒状杆菌(C.commune)。结论 MALDI-TOF MS可将棒状杆菌属细菌快速、准确地鉴定到种。

引用

页码：668 / 670

页数：3

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