机体炎症因子和氧化应激标志物介导姜黄素抑制骨性关节炎的作用机制

被引：13

作者：

刘军 ^{[1
]}

何晓乐 ^{[2
]}

甄平 ^{[1
]}

周胜虎 ^{[1
]}

李旭升 ^{[1
]}

机构：

[1] 兰州军区兰州总医院全军骨科中心关节外科

[2] 第四军医大学西京医院老年病科

来源：

浙江大学学报(医学版) | 2016年 / 05期

关键词：

骨关节炎/病理学; 姜黄素/药理学; 姜黄素/投药和剂量; 基质金属蛋白酶2; 趋化因子CCL2; 抗原,CD47; L选择素; 蛋白水解产物; 疾病模型,动物;

D O I：

暂无

中图分类号：

R684.3 [关节炎];

学科分类号：

100220 [骨科学];

摘要：

目的:探讨骨性关节炎(OA)关节软骨基质金属蛋白酶-2(MMP-2)、单核细胞趋化蛋白-1(MCP-1)、CD47、L-选择素及高级氧化蛋白产物(AOPP)表达的变化以及姜黄素(Cur)的干预作用。方法:建立C57BL/6小鼠OA模型,20只小鼠随机分为手术对照组、模型对照组、Cur25组和Cur50组(术后分别每日接受25μmol/L和50μmol/L浓度姜黄素腹腔注射)。4周后取材,采用免疫组织化学法观察各组大体标本形态学变化,电子显微镜下观察软骨组织超微结构;培养各组软骨细胞,运用蛋白质印迹法检测各组MMP-2、MCP-1和CD47表达,ELISA和分光光度法检测L-选择素水平及AOPP浓度变化。结果:软骨组织标本形态学变化模型对照组最明显,Cur25组次之;与手术对照组比较,模型对照组、Cur25组、Cur50组软骨细胞MMP-2、MCP-1表达均增多(均P<0.05),CD47表达均下降(均P<0.05),L-选择素水平及AOPP浓度均增加(均P<0.05);与模型对照组比较,Cur25组和Cur50组软骨细胞MMP-2、MCP-1、L-选择素及AOPP的表达下降(均P<0.05),CD47的表达上升(均P 更多还原 AbstractFilter('ChDivSummary', 'ChDivSummaryMore', 'ChDivSummaryReset');

引用

页码：461 / 468

页数：8

共 10 条

[1]

Atlas of radiographic features of osteoarthritis of the ankle and hindfoot[J] V.B. Kraus;T.M. Kilfoil;T.W. Hash;G. McDaniel;J.B. Renner;J.A. Carrino;S. Adams Osteoarthritis and Cartilage 2015,

[2]

Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazone[J] Y. Li;Y. Zhang;C. Chen;H. Zhang;C. Ma;Y. Xia Osteoarthritis and Cartilage 2016,

[3]

Prospective change in daily walking over 2 years in older adults with or at risk of knee osteoarthritis: the MOST study[J] D.K. White;C. Tudor-Locke;Y. Zhang;J. Niu;D.T. Felson;K.D. Gross;M.C. Nevitt;C.E. Lewis;J. Torner;T. Neogi Osteoarthritis and Cartilage 2016,

[4]

Evidence that meniscus damage may be a component of osteoarthritis: the Framingham study[J] M. Englund;I.K. Haugen;A. Guermazi;F.W. Roemer;J. Niu;T. Neogi;P. Aliabadi;D.T. Felson Osteoarthritis and Cartilage 2016,

[5]

Quantitative measures of meniscus extrusion predict incident radiographic knee osteoarthritis – data from the Osteoarthritis Initiative[J] K. Emmanuel;E. Quinn;J. Niu;A. Guermazi;F. Roemer;W. Wirth;F. Eckstein;D. Felson Osteoarthritis and Cartilage 2016,

[6]

Short-term effects of highly-bioavailable curcumin for treating knee osteoarthritis: a randomized; double-blind; placebo-controlled prospective study[J] Yasuaki Nakagawa;Shogo Mukai;Shigeru Yamada;Masayuki Matsuoka;Eri Tarumi;Tadashi Hashimoto;Chieko Tamura;Atsushi Imaizumi;Jun Nishihira;Takashi Nakamura Journal of Orthopaedic Science 2014,

[7]

Curcumin: a new paradigm and therapeutic opportunity for the treatment of osteoarthritis: curcumin for osteoarthritis management[J] Yves Henrotin;Fabian Priem;Ali Mobasheri SpringerPlus 2013,

[8]

Synovial tissue–infiltrating natural killer cells in osteoarthritis and periprosthetic inflammation[J] Ryan S.Huss;James I.Huddleston;Stuart B.Goodman;Eugene C.Butcher;Brian A.Zabel Arthritis & Rheumatism 2010,

[9]

Antidepressant properties of the 5-HT 4 receptor partial agonist; SL65.0155: Behavioral and neurochemical studies in rats[J] Alessandra Tamburella;Vincenzo Micale;Andrea Navarria;Filippo Drago Progress in Neuropsychopharmacology & Biological Psychiatry 2009,

[10]

Innate immune ‘self’ recognition: a role for CD47–SIRPα interactions in hematopoietic stem cell transplantation[J] Timo K. van den Berg;C. Ellen van der Schoot Trends in Immunology 2008,

← 1 →