应用DPO引物检测马铃薯病毒的多重RT-PCR技术研究

被引：27

作者：

刘梅 ^{[1
,2
]}

黄新 ^{[2
]}

马占鸿 ^{[1
]}

陈洪俊 ^{[2
]}

李明福 ^{[2
]}

机构：

[1] 中国农业大学农学与生物技术学院

[2] 中国检验检疫科学研究院

来源：

植物病理学报 | 2009年 / 04期

关键词：

dual priming oligonucleotide(DPO) primers; multiplex RT-PCR; potato viruses;

D O I：

10.13926/j.cnki.apps.2009.04.008

中图分类号：

S435.32 [马铃薯（土豆）病虫害];

学科分类号：

090401 ; 090402 ;

摘要：

DPO is one of the new specific primers for virus detection.In this study,five sets of dual priming oligonucleotide(DPO) primers for Potato virus X(PVX),Potato virus Y(PVY),Potato virus V(PVV),Tobacco ring spot virus(TRSV) and Cucumber mosaic virus(CMV) were designed and applied to the detection of the main potato viruses with multiplex reverse transcription polymerase chain reaction(m-RT-PCR),including two quarantine viruses in China(PVV and TRSV).The results showed that DPO-m-RT-PCR exhi-bited high PCR specificity and sensitivity even under less than optimal PCR condition compared with conventional m-RT-PCR.DPO-m-RT-PCR could be applied in the healthy evaluation of seed potato and its exit-entry quarantine.

引用

页码：431 / 434

页数：4

共 2 条

[1] Direct detection of lamivudine-resistant hepatitis B virus mutants by a multiplex PCR using dual-priming oligonucleotide primers [J].