细菌人工染色体文库的构建及应用

被引：10

作者：

王晓虹 ^{[1
]}

金黎明 ^{[2
]}

机构：

[1] 宝生物工程(大连)有限公司

[2] 大连民族学院生命科学学院

来源：

生物技术通讯 | 2005年 / 06期

关键词：

细菌人工染色体; 文库构建; 鉴定; 应用;

D O I：

暂无

中图分类号：

Q78 [基因工程（遗传工程）];

学科分类号：

071007 ; 0836 ; 090102 ;

摘要：

细菌人工染色体(BAC)是第二代大片段DNA的克隆载体系统,具有容量大、嵌合率低、遗传特性稳定、转化效率高、插入片段易回收、操作简便等优点,因而被广泛应用于基因组较大的真核生物基因组研究中,并发挥着前所未有的重要作用。本文综述了BAC的发展,利用此载体构建基因组文库的程序和鉴定方法,及其在物理图谱构建、图位克隆、基因组测序、转基因技术等研究中的应用。

引用

页码：668 / 671

页数：4

共 6 条

[1]

Construction of a BAC library of pearl millet, Pennisetum glaucum[J] . S. Allouis,X. Qi,S. Lindup,M. D. Gale,K. M. Devos. &nbspTAG Theoretical and Applied Genetics . 2001 (8)

[2]

Construction of a bacterial artificial chromosome library containing large Eco RI and Hin dIII genomic fragments of lettuce[J] . A. C. J. Frijters,Z. Zhang,M. van Damme,G.-L. Wang,P. C. Ronald,R. W. Michelmore. &nbspTAG Theoretical and Applied Genetics . 1997 (3-4)

[3] Construction and characterization of two rice bacterial artificial chromosome libraries from the parents of a permanent recombinant inbred mapping population [J].

Zhang, HB ;

Choi, SD ;

Woo, SS ;

Li, ZK ;

Wing, RA .

MOLECULAR BREEDING, 1996, 2 (01) :11-24

[4] A bacterial artificial chromosome library for soybean and identification of clones near a major cyst nematode resistance gene [J].

Danesh, D ;

Peñuela, S ;

Mudge, J ;

Denny, RL ;

Nordstrom, H ;

Martinez, JP ;

Young, ND .

THEORETICAL AND APPLIED GENETICS, 1998, 96 (02) :196-202

[5]

Construction of a bacterial artificial chromosome library of Medicago truncatula and identification of clones containing ethylene-response genes[J] . Y.-W. Nam,R. V. Penmetsa,G. Endre,P. Uribe,D. Kim,D. R. Cook. &nbspTAG Theoretical and Applied Genetics . 1999 (3-4)

[6]

Generation of a soybean BAC library, and identification of DNA sequences tightly linked to the Rps1-k disease resistance gene[J] . S. S. Salimath,M. K. Bhattacharyya. &nbspTAG Theoretical and Applied Genetics . 1999 (5)

← 1 →