双价抗虫基因叶绿体共转化植株抗虫性及其后代表型分析

被引:12
作者
苏宁
孙萌
杨波
孟昆
刘春英
倪丕冲
沈桂芳
机构
[1] 中国农业科学院生物技术研究所叶绿体基因工程实验室,浙江大学生命科学院,中国农业科学院生物技术研究所叶绿体基因工程实验室,中国农业科学院生物技术研究所叶绿体基因工程实验室,中国农业科学院生物技术研究所叶绿体基因工程实验室,中国农业科学院生物技术研究所叶绿体基因工
关键词
OC基因; Bt基因; 叶绿体转化; 抗虫作用;
D O I
10.16288/j.yczz.2002.03.016
中图分类号
Q943.2 [植物基因工程];
学科分类号
071007 ; 090102 ;
摘要
利用基因枪法将含有水稻巯基蛋白酶抑制剂 (Oryzacystatin ,OC)基因烟草叶绿体表达载体和含有苏云金芽孢杆菌晶体毒蛋白基因 (BtcryIAc)烟草叶绿体表达载体 ,共转化烟草叶绿体 ,获得壮观霉素抗性植株。转基因植株抗棉铃虫试验表明 ,转双价抗虫基因植株比转单一抗虫基因植株具有更强的杀虫活性。转基因植株后代Southern检测及其遗传学分析试验证明 ,双价抗虫基因可以稳定地遗传给后代 ,且表现为叶绿体特有的母系遗传规律。
引用
收藏
页码:288 / 292
页数:5
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