SEREX方法鉴定胃癌肿瘤相关抗原基因

被引：5

作者：

王运伟

朱正纲

顾琴龙

李建芳

陈雪华

刘炳亚

林言箴

机构：

[1] 上海第二医科大学瑞金医院外科上海消化外科研究所

[2] 上海第二医科大学瑞金医院外科上海消化外科研究所上海

[3] 上海

来源：

上海第二医科大学学报 | 2005年 / 03期

基金：

国家高技术研究发展计划(863计划);

关键词：

胃癌; cDNA文库; 重组cDNA表达文库血清学分析法; 肿瘤抗原;

D O I：

暂无

中图分类号：

R735.2 [胃肿瘤];

学科分类号：

100214 ;

摘要：

目的采用重组CDNA表达文库血清学分析法(SEREX法)寻找胃癌相关肿瘤抗原的编码基因。方法抽提胃癌细胞株SGC7901和1例胃癌组织标本PolyA+RNA,分别构建2个胃癌cDNA表达文库,用含有抗体的胃癌患者血清对2个文库进行筛选,并对筛选所得到的阳性克隆进行测序和生物信息学分析。结果经测定2个原始文库滴度分别为1.6×106pfu和2.5×106pfu,重组率为95%和98%。2个文库经3轮筛选共获得14个胃癌肿瘤相关抗原编码基因,其中一个为未知基因。13个已知基因编码产物包括代谢酶、RNA转录和翻译调控蛋白、蛋白代谢相关分子、自身免疫疾病相关抗原及未知功能蛋白分子。结论　胃癌相关肿瘤抗原编码基因的筛选和鉴定,为胃癌的临床诊断和特异性免疫治疗提供了靶点,有助于对胃癌发生机制的深入理解。

引用

页码：235 / 238

页数：4

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