HPLC-MS测定黄芪药材中3种成分的含量

被引：30

作者：

李翔 ^{[1
]}

朱臻宇 ^{[1
]}

王彬 ^{[2
]}

娄子洋 ^{[1
]}

柴逸峰 ^{[1
]}

机构：

[1] 第二军医大学药学院

[2] 东方肝胆外科医院药剂科

来源：

药学学报 | 2006年 / 08期

关键词：

高效液相色谱-质谱联用; 黄芪药材; 毛蕊异黄酮苷; 黄芪甲苷; 芒柄花素;

D O I：

暂无

中图分类号：

R284 [中药化学];

学科分类号：

100804 [中药化学];

摘要：

Aim To determine calycosin-7-O-β-D-glucoside,astragaloside IV and formononetin in Radix Astragali and other relative samples by HPLC-MS.Methods HPLC was carried out with Agilent 1100LC/MSD,equipped with Agilent Zorbax SB C18 column（250 mm×4.6 mm ID,5 μm） and mass spectrum detector.The mobile phase（CH3CN-H2O） was eluted in gradient mode.Results The calibration curves of calycosin-7-O-β-D-glucoside,astragaloside IV and formononetin were linear in the range of 0.031.21 μg·mL-1,0.35-13.86 μg·mL-1 and 0.38-15.22 μg·mL-1,respectively.These recoveries of samples were from 95% to 105% with RSD less than 1.5%.Conclusion The method was employed to analyse 25 samples of Radix Astragali and other relative samples,including Radix Astragali slice,Radix Astragali Preparata,Hedysarum polybotrys Hand.-Mazz,Astragalus ernestii Comb.The contents of three constituents vary greatly because of the species, place of collection and season of harvesting.This method could apply to evaluate the quality of Radix Astragali and it is simple,sensitive and reliable.

引用

页码：793 / 796

页数：4