用差异显示PCR技术克隆冬小麦春化设定cDNA克隆实验系统的建立

被引:5
作者
赵大中
陈民
谭克辉
机构
[1] 北京大学生命科学学院细胞生物学与遗传学系,中国科学院植物研究所发育室,中国科学院植物研究所发育室北京,北京,北京
关键词
差异显示PCR; 春化设定cDNA克隆; 冬小麦;
D O I
暂无
中图分类号
S512.1 [小麦];
学科分类号
摘要
差异显示PCR技术是一种新近发展起来的在真核细胞中检测与克隆特异表达基因的手段。本文建立了用此技术克隆春化相关基因的研究系统,并提供了诸如总RNA的提取、污染DNA的去除、sscDNA的逆转录、PCR参数、扩增cDNA的电泳、特异cDNA的收集与重扩增等在方法上的细节。用这种技术分离了一个仅在春化20天这一特异时期表达的春化设定cDNA克隆VPC28。这些结果也适用于更加广泛的类似研究。
引用
收藏
页码:47 / 54
页数:8
相关论文
共 5 条
[1]  
Identification of mRNAs with enhanced expression in ripening strawberry fruit using polymerase chain reaction differential display[J] . Jack Q. Wilkinson,Michael B. Lanahan,Timothy W. Conner,Harry J. Klee. &nbspPlant Molecular Biology . 1995 (6)
[2]   ISOLATION OF A NOVEL ARABIDOPSIS OZONE-INDUCED CDNA BY DIFFERENTIAL DISPLAY [J].
SHARMA, YK ;
DAVIS, KR .
PLANT MOLECULAR BIOLOGY, 1995, 29 (01) :91-98
[3]  
Identification of a gibberellin-induced gene in deepwater rice using differential display of mRNA[J] . Esther Knaap,Hans Kende. &nbspPlant Molecular Biology . 1995 (3)
[4]   PATTERNS OF GENE-EXPRESSION IN DEVELOPING ANTHERS OF BRASSICA-NAPUS [J].
SCOTT, R ;
DAGLESS, E ;
HODGE, R ;
PAUL, W ;
SOUFLERI, I ;
DRAPER, J .
PLANT MOLECULAR BIOLOGY, 1991, 17 (02) :195-207
[5]   Application of modified differential display technique for cloning and sequencing of the 3' region from three putative members of wheat HSP70 gene family [J].
Joshi, CP ;
Kumar, S ;
Nguyen, HT .
PLANT MOLECULAR BIOLOGY, 1996, 30 (03) :641-646