蛋白质酶法有限水解过程反应机理和动力学特性

被引：19

作者：

刘璘

叶峰

欧志敏

机构：

[1] 浙江工业大学生物与环境工程学院

[2] 浙江工业大学生物与环境工程学院浙江杭州

[3] 浙江杭州

来源：

化工学报 | 2002年 / 02期

关键词：

大豆分离蛋白; 蛋白酶; 有限水解; 反应机理; 动力学;

D O I：

暂无

中图分类号：

TQ93 [蛋白质（朊）化学加工工业];

学科分类号：

081703 ;

摘要：

The enzymatic limited hydrolysis of soy protein isolate (SPI) by Alcalase 0.6?L was carried out with pH-stat method at pH 8.0,50?℃.The effects of the main parameters on the degree of hydrolysis, x , were investigated and a model based on the experiment results was deduced.The results showed that the substrate and the enzyme-substrate intermediate induced enzyme inhibition,which occurred due to free enzyme attacking the enzyme-substrate complexes.A critical 0 would correspond to a constant 0,which was 96.77?mg·ml -1 under the experiment condition.The kinetic parameters calculated from the experiment data were K m=0.016?mol·L -1 and V max =0.765?mol·min·L -1 .The results showed that x can be regulated to about 6.0 with the mentioned factors in 30?min.The good fitness between predicted and measured results confirmed the usefulness of the model.

引用

页码：199 / 202

页数：4