失神经支配兔下颌骨骨折愈合过程中CGRP对OPG/RANKL表达的影响

被引：17

作者：

徐琳

谭颖徽

王建华

张纲

机构：

[1] 第三军医大学新桥医院口腔科

[2] 第三军医大学新桥医院口腔科重庆

[3] 重庆

来源：

第三军医大学学报 | 2005年 / 10期

关键词：

降钙素基因相关肽(CGRP); 骨保护素(OPG); 破骨细胞分化因子(RANKL)失神经支配; 下颌骨骨折;

D O I：

10.16016/j.1000-5404.2005.10.023

中图分类号：

R782.4 [口腔颌面部损伤];

学科分类号：

100302 ;

摘要：

目的　明了失神经支配兔下颌骨骨折愈合过程中CGRP对OPG/RANKL表达的影响及其调节骨折愈合的机制。方法　40只新西兰白兔随机分为:单纯下颌骨骨折组及下齿槽神经离断+下颌骨骨折组。分别于骨折术后7、14、2 1、2 8d处死,取骨痂标本行HE及免疫组织化学染色。结果　单纯骨折组骨折后7d骨痂中即可见到降钙素基因相关肽(CGRP)阳性神经纤维,沿血管分布,14、2 1d在编织骨边缘有大量的CGRP阳性神经纤维,2 8d仍较多。同时骨折后7d骨保护素(OPG)强阳性表达,以后逐渐下降,但均保持较高水平的表达。破骨细胞分化因子(RANKL)的表达高峰则在骨折后第14天;而失神经骨折组除7d骨痂中见CGRP阳性表达外,7d后骨痂中仅有极低水平的CGRP表达。同样,OPG在骨折后7d在骨痂中阳性表达以后持续弱阳性表达。RANKL则持续强阳性表达。结论　在骨折愈合过程中,CGRP能够上调OPG/RANKL的表达量的比值,从而参与骨折愈合过程的调节,失神经支配失去了CGRP对OPG/RANKL表达的调节作用,不利于骨折愈合。

引用

页码：988 / 990

页数：3

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