枯草芽孢杆菌B2菌株产生的抑菌活性物质分析

被引:60
作者
高学文
姚仕义
Huong Pham
Joachim Vater
王金生
机构
[1] 南京农业大学农业部病虫监测与治理重点开放实验室
[2] 柏林理工大学生物化学和分子生物学研究所
[3] 南京农业大学农业部病虫监测与治理重点开放实验室 南京 
[4] 南京 
关键词
枯草芽孢杆菌; HPLC; 分离纯化; LCMS分析; 抑制活性;
D O I
10.16409/j.cnki.2095-039x.2003.04.008
中图分类号
S476 [生物防治];
学科分类号
摘要
枯草芽孢杆菌B2菌株产生的胞外物质经盐酸沉淀,甲醇抽提获得粗制备物。利用HPLC系统将粗制备物过XterraRP18层析柱分离,共获得120管收集液。以抑制小麦赤霉病菌分生孢子萌发为指标对各管收集液的抑菌活性进行了测定。通过LC MS分析,结果表明B2菌株胞外存在3种抑菌物质,即脂肽类抗生素表面活性素、多烯类和一种分子量为564的结构未知的新物质。
引用
收藏
页码:175 / 179
页数:5
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