青花菜查尔酮合成酶基因BoCHS的克隆、表达及序列分析

被引：4

作者：

彭礼琼 ^{[1
]}

蒋明 ^{[1
]}

郭志平 ^{[2
]}

章鲤静 ^{[1
]}

张徐俞 ^{[1
]}

机构：

[1] 台州学院生命科学学院

[2] 丽水学院化学与生命科学学院

来源：

湖北农业科学 | 2010年 / 49卷 / 08期

基金：

浙江省自然科学基金;

关键词：

青花菜; 查尔酮合成酶基因; 序列分析; 基因表达;

D O I：

10.14088/j.cnki.issn0439-8114.2010.08.049

中图分类号：

S635.3 [花椰菜];

学科分类号：

摘要：

根据NCBI数据库中发布的查尔酮合成酶基因序列设计PCR简并引物,以青花菜(Brassica oler-acea var.italica)子叶cDNA为材料,克隆到了青花菜查尔酮合成酶基因,基因命名为BoCHS,序列已提交到NCBI数据库,登录号为GU266209。该基因的编码区全长为1182bp,编码393个氨基酸。序列比对结果表明,BoCHS基因编码的氨基酸序列与其他十字花科植物之间的同源性很高,仅存在个别氨基酸残基的差别。RT-PCR检测表明,经霜霉病菌(Hyaloperonospora parasitica)侵染后,子叶中BoCHS的表达量增加,其中以72h和96h的表达量最大。

引用

页码：1796 / 1800

页数：5

共 7 条

[1] Sequence variation of chalcone synthase gene in a spontaneous white-flower mutant of Chinese cabbage-pak-choi [J].

Jiang, Ming ;

Cao, Jiashu .

MOLECULAR BIOLOGY REPORTS, 2008, 35 (04) :507-512

[2] Evidence that a leaf-disk test allows assessment of isolate-specific resistance in Brassica oleracea crops against downy mildew (Peronospora parasitica) [J].

Agnola, B ;

Boury, S ;

Monot, C ;

Quillévéré, A ;

Hervé, Y ;

Silué, D .

EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2003, 109 (05) :471-478

[3] Chalcone synthase and phenylalanine ammonia-lyase mRNA levels following exposure of sorghum seedlings to three fungal pathogens [J].

Cui, Y ;

Magill, J ;

Frederiksen, R ;

Magill, C .

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1996, 49 (03) :187-199

[4]

Isolation and sequence analysis of a chalcone synthase cDNA of Matthiola incana R. Br. (Brassicaceae)[J] . B. Epping,M. Kittel,B. Ruhnau,V. Hemleben.Plant Molecular Biology . 1990 (6)

[5]

Phytoalexin Detoxification: Importance for Pathogenicity and Practical Implications[J] . H D VanEllen,D E Matthews,P S Matthews.Annual Review of Phytopathology . 1989

[6]

Nucleotide sequences encoding two different chalcone synthases expressed in cotyledons of SAN 9789 treated mustard ( Sinapis alba L.)[J] . Plant Molecular Biology . 1988 (6)

[7]

Rapid induction of phenylalanine ammonia-lyase and chalcone synthase mRNAs during fungus infection of soybean ( Glycine max L.) roots or elicitor treatment of soybean cell cultures at the onset of phytoalexin synthesis[J] . Planta . 1989 (1)

← 1 →