ISSR和RAPD-PCR技术对东北地区主推玉米品种的鉴别

被引：9

作者：

李尚禹 ^{[1
]}

王春艳 ^{[2
]}

张朝燕 ^{[3
]}

李校堃 ^{[4
]}

机构：

[1] 长春市产品质量监督检验院

[2] 吉林大学公共卫生学院

[3] 长春师范学院生物系

[4] 吉林农业大学生物工程技术研究院

来源：

吉林大学学报(理学版) | 2006年 / 03期

关键词：

玉米; ISSR; RAPD; 品种鉴别;

D O I：

10.13413/j.cnki.jdxblxb.2006.03.043

中图分类号：

S513 [玉米（玉蜀黍）];

学科分类号：

摘要：

运用现代分子生物学简单重复区间序列扩增多态性(ISSR)和随机扩增片段多态性DNA(RAPD)两种分子标记技术,对吉林省主推6个玉米杂交种及其父本、母本共计18份基因图谱进行鉴别.以ISSR为主要检测方法、RAPD为验证方法,分别从30条ISSR引物中选出4条、从30条RAPD引物中筛选出2条扩增效果明显、特异性高的引物.结果表明,运用ISSR和RAPD-PCR技术提高了玉米品种鉴定和纯度鉴定的准确性.

引用

页码：509 / 512

页数：4

共 7 条

[1]

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[2]

ARapid and Simple Method for the Preparation of Plant Genomic DNAfor PCR Analysis. Edwards K. Nucleic Acids Research . 1991

[3]

Variation in Inter-simple Sequence Repeat(ISSR)in Mangrove and Non-mangrove Populations of Heritiera Littoralis(Sterculiaceae)from China and Australia. JIAN Shu-Guang,TANG Tian. Aquatic Botany . 2004

[4]

Genetic Diversity among and within Populations of Oryza Granulata from Yunnan of China Revealed by RAPD and ISSR Markers:Implications for Conservation of the Endangered Species. WU Cheng-jun,CHENG Zai-quan. Plant Science . 2004

[5]

[6]

The Concordance of Terpenoid,ISSR and RAPD Markers and Its Sequence Data Sets among Genotypes:an Example from Juniperus. Adams Robert P,Schwarzbach Andrea E. Biochemical Systematics and Ecology . 2003

[7]

Genome Fingerprinting by Simple Sequence Repeat(SSR)-anchored Polymerase Chain Reaction Amplification. Zietkiewicz E. Genome . 1994

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