花粉介导的转Bt基因棉花田间基因流监测

被引：13

作者：

王长永 ^{[1
]}

刘燕 ^{[2
]}

周骏 ^{[1
]}

陈建群 ^{[1
]}

钦佩 ^{[1
]}

机构：

[1] 南京大学生命科学学院

[2] 国家环境保护总局南京环境科学研究所

来源：

应用生态学报 | 2007年 / 04期

关键词：

转Bt基因棉花; 基因流; 花粉漂移; 花粉粒染色法; 监测;

D O I：

10.13287/j.1001-9332.2007.0135

中图分类号：

S562 [棉];

学科分类号：

摘要：

采用花粉粒染色法对转Bt基因棉的花粉漂移距离和强度进行了观测,并应用PCR法检测转Bt基因棉的基因流频率.花粉粒染色法监测结果表明:同株异花间的花粉散布频率显著高于异株异花间(P<0.01);靠近转Bt基因棉花粉染色区1m处的平均花粉散布频率,在东、南、西、北4个方向分别为44.8%、48.9%、57.1%和21.5%,但随着距转基因棉田距离的增大,4个方向的平均花粉散布频率都呈下降趋势.PCR结果的统计分析表明,在25m内,花粉散布距离和方向对基因流频率有极显著影响(P<0.01),随着距转基因棉田距离的增大,基因流频率呈下降趋势,最远距离为25m时的最高基因流频率为2.0%.

引用

页码：801 / 806

页数：6

共 8 条

[1]

Out-crossing frequency and genetic analysis of hybrids between transgenic glufosinate herbicide-resistant rice and the weed, red rice[J] . Nengyi Zhang,Steve Linscombe,James Oard.Euphytica . 2003 (1)

[2]

Field assessments of gene flow from transgenic to cultivated rice （Oryza sativa L.） using a herbicide resistance gene as tracer marker[J] . J. Messeguer,C. Fogher,E. Guiderdoni,V. Marfà,M. M. Català,G. Baldi,E. Melé.TAG Theoretical and Applied Genetics . 2001 (8)

[3] Assessment of interspecific hybridization between transgenic oilseed rape and wild radish under normal agronomic conditions [J].

Chèvre, AM ;

Eber, F ;

Darmency, H ;

Fleury, A ;

Picault, H ;

Letanneur, JC ;

Renard, M .

THEORETICAL AND APPLIED GENETICS, 2000, 100 (08) :1233-1239

[4]

Pollen dispersal from two field trials of transgenic cotton in the Namoi Valley, Australia[J] . Danny Llewellyn,Gary Fitt.Molecular Breeding . 1996 (2)

[5]

Inheritance of oilseed rape （ Brassica napus ） RAPD markers in a backcross progeny with Brassica campestris[J] . Theoretical and Applied Genetics . 1996 (3)

[6]

Effect of oilseed rape genotype on the spontaneous hybridization rate with a weedy species:an assessment of transgene dispersal[J] . A. Baranger,A. M. Chèvre,F. Eber,M. Renard.Theoretical and Applied Genetics . 1995 (6)

[7]

Biological containment of potato （Solanum tuberosum） : outcrossing to the related wild species black nightshade （Solanum nigrum） and bittersweet （Solanum dulcamara）[J] . Ronald Eijlander,Willem J. Stiekema.Sexual Plant Reproduction . 1994 (1)

[8]

The use of neutron activation analysis in pollination ecology[J] . Martha M. Gaudreau,James W. Hardin.Brittonia . 1974 (3)

← 1 →