免疫刺激DNA序列与过敏原联用对哮喘小鼠模型气道过敏性炎症的作用

被引：2

作者：

金美玲

蔡映云

袁正宏

郑玲洁

任涛

机构：

[1] 复旦大学附属中山医院呼吸科,复旦大学附属中山医院呼吸科,复旦大学医学院分子病毒学实验室,复旦大学医学院分子病毒学实验室,复旦大学附属中山医院呼吸科上海,上海,上海

来源：

中华结核和呼吸杂志 | 2002年 / 09期

关键词：

哮喘; 免疫刺激DNA序列; 气道过敏性炎症;

D O I：

暂无

中图分类号：

R562.25 [];

学科分类号：

1002 ; 100201 ;

摘要：

目的　观察免疫刺激DNA序列 (ISS DNA)单用和与过敏原卵白蛋白 (OVA)合用 ,对支气管哮喘 (简称哮喘 )小鼠模型气道过敏性炎症的作用及作用维持时间。方法　BALB/c小鼠 36只 ,分ISS组 (A组 ) 12只、ISS +OVA组 (B组 ) 12只、OVA组 (C组 ) 6只和生理盐水 (NS)组 (D组 ) 6只。A、B、C组用OVA致敏及激发。A组和B组根据注射次数再分A1、B11次注射组 (1次腹腔注射ISS DNA 10 0μg或ISS DNA 10 0 μg +OVA 10 μg)和A2 、B2 2次注射组 (2次腹腔注射ISS DNA或ISS DNA +OVA)。各组在第 1次激发后 6周中 ,每周采血 1次测特异性IgE ,最后处死小鼠测支气管肺泡灌洗液 (BALF)中细胞计数及分类 ,肺组织病理检查 ,检测脾细胞分泌γ干扰素 (IFN γ)的水平。结果　BALF中嗜酸细胞 (EOS)数A1组为 (2 39± 0 81)× 10 4/ml;A2 组为 (2 .6 2± 0 .77)× 10 4/ml;B1组为 (1.80± 0 .12 )× 10 4/ml;B2 组为 (1.84± 0 .6 7)× 10 4/ml,与C组 [(12 .4 3± 2 .13)× 10 4/ml]比较差异有显著性 (P <0 .0 5 )。脾细胞分泌的IFN γA1组为 (5 10± 10 2 )pg/ml;A2 组为 (492± 98)pg/ml;B1组为 (5 32± 12 0 )pg/ml;B2组为 (46 9± 132 )pg/ml,与C组 [(194± 80 )pg/ml]比较差异有显著性 (P <0 .0 5 )。血清IgE前 4周B组与C组比较

引用

页码：30 / 33+66

页数：5

共 12 条

[1]

Sorting out the cytokines of asthma. Drazen JM,Arm JP,Austen KF. The Journal of Experimental Medicine . 1996

[2]

Interleukin 4, but not interleukin 5 or eosinophils, is required in a murine model of acute airway hyperreactivity. Corry DB,Folkesson HG,Warnock ML,et al. The Journal of Experimental Medicine . 1996

[3]

DNA-based immunization for asthma. Broide D,Raz E. International Archives of Allergy and Immunology . 1999

[4]

Immunostimulatory DNA sequences necessary for effective intradermal gene immunization. Sato Y,Roman M,Tighe H,et al. Science . 1996

[5]

CpG motifs present in bacteria DNA rapidly induce lymphocytes to secrete interleukin 6,interleukin 12, and interferon-gamma. Klinman DM,Yi AK,Beaucage SL,et al. Proceedings of the National Academy of Sciences of the United States of America . 1996

[6]

Regulation of murine airway eosinophilia and Th2 cells by antigen-conjugated CpG oligodeoxynucleotides as a novel antigen-specific immunomodulator. Shirota H,Sano K,Kikuchi T,et al. J Immunol . 2000

[7]

Regulation of T-helper type 2 cell and airway eosinophilia by transmucosal coadministration of antigen and oligodeoxynucleotides containing CpG motifs. Shirota H,Sano K,Kikuchi T,et al. Am J Respir Cell Mol Biol . 2000

[8]

Allergic and nonallergic asthmatics have distinct patterns of T-cell activation and cytokine production in peripheral blood and bronchoalveolar lavage. Walker C,Bode E,Boer L,et al. The American Review of Respiratory Disease . 1992

[9]

Preferential induction of a Th1 immune response and inhibition of specific IgE antibody formation by plasmid DNA immunization. Raz E,Tighe H,Sato Y,et al. Proceedings of the National Academy of Sciences of the United States of America . 1996

[10]

Modulation of airway inflammation by CpG oligodeoxynucleotides in a murine model of asthma. Kline JN,Waldschmidt TJ,Businga TR,et al. J Immunol . 1998

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