青钱柳三萜酸对高糖所致的胰岛α细胞胰岛素抵抗的影响

被引：34

作者：

王依婷 ^{[1
,2
,3
]}

赵梦鸽 ^{[1
,2
,3
]}

盛雪萍 ^{[1
,2
,3
]}

张健 ^{[2
,3
]}

蒋翠花 ^{[2
,3
]}

殷志琦 ^{[1
]}

机构：

[1] 中国药科大学天然药物化学教研室&天然药物活性组分与药效国家重点实验室

[2] 南京中医药大学附属中西医结合医院

[3] 江苏省中医药研究院转化医学实验室

来源：

中国药科大学学报 | 2018年 / 49卷 / 02期

关键词：

青钱柳; 三萜酸; αTC1-6细胞; 胰岛素抵抗; 2型糖尿病;

D O I：

暂无

中图分类号：

R285 [中药药理学];

学科分类号：

100806 [中药药理学];

摘要：

为探究青钱柳三萜酸(TAE)对胰岛素抵抗胰岛α细胞胰高血糖素分泌的影响及其可能的干预机制,采用长期高糖诱导小鼠胰高糖素瘤细胞株(αTC1-6)建立胰岛α细胞胰岛素抵抗细胞模型。实验分组为:正常对照组(5.5 mmol/L葡萄糖),模型组(25 mmol/L葡萄糖),青钱柳三萜酸组(TAE,1、5、10μg/mL),TAE+PI3K抑制剂渥曼青霉素组(10μg/mL TAE+10 nmol/L wortmannin)。给药后收集上清液,裂解细胞。采用ELISA试剂盒检测αTC1-6细胞上清液的胰高血糖素含量,q PCR和Western blot检测细胞裂解液中的胰岛素受体-1(IRS-1)、磷脂酰肌醇3激酶(PI3K)及其胰岛素信号通路中下游蛋白激B(Akt)的m RNA和蛋白表达水平。结果显示,TAE可以浓度依赖性降低高糖诱导的αTC1-6细胞的胰高血糖素分泌量,并可增加胞内IRS-1、PI3K和Akt的基因表达和磷酸化水平,而给予渥曼青霉素后能够逆转其作用。研究表明,TAE对高糖所致的胰岛α细胞胰岛素抵抗具有一定的改善作用,并可能与激活IRS/PI3K/Akt信号通路有关。

引用

页码：215 / 221

页数：7

共 12 条

[1]

Two triterpeniods from Cyclocarya paliurus (Batal) Iljinsk (Juglandaceae) promote glucose uptake in 3T3-L1 adipocytes: The relationship to AMPK activation [J].

Zhu, K. N. ;

Jiang, C. H. ;

Tian, Y. S. ;

Xiao, N. ;

Wu, Z. F. ;

Ma, Y. L. ;

Lin, Z. ;

Fang, S. Z. ;

Shang, X. L. ;

Liu, K. ;

Zhang, J. ;

Liu, B. L. ;

Yin, Z. Q. .

PHYTOMEDICINE, 2015, 22 (09) :837-846

[2]

Antihyperglycemic, antihyperlipidemic and antioxidant effects of ethanol and aqueous extracts of Cyclocarya paliurus leaves in type 2 diabetic rats [J].

Wang, Qingqing ;

Jiang, Cuihua ;

Fang, Shengzuo ;

Wang, Junhu ;

Ji, Yun ;

Shang, Xulan ;

Ni, Yicheng ;

Yin, Zhiqi ;

Zhang, Jian .

JOURNAL OF ETHNOPHARMACOLOGY, 2013, 150 (03) :1119-1127

[3]

Glucagon: The renewal of an old hormone in the pathophysiology of diabetes (胰升糖素：一种老激素在糖尿病病理生理中的新作用).[J].Yvan Gosmain;Mounia Heddad Masson;Jacques Philippe.Journal of Diabetes.2013, 2

[4]

Glucotoxicity and α Cell Dysfunction: Involvement of the PI3K/Akt Pathway in Glucose-Induced Insulin Resistance in Rat Islets and Clonal αTC1-6 Cells [J].

Shen, Xiao-xia ;

Li, Hong-liang ;

Pan, Lin ;

Hong, Jing ;

Xiao, Juan ;

Hermansen, Kjeld ;

Jeppesen, Per Bendix ;

Li, Guang-Wei .

ENDOCRINE RESEARCH, 2012, 37 (01) :12-24

[5]

Glucagonocentric restructuring of diabetes: a pathophysiologic and therapeutic makeover [J].

Unger, Roger H. ;

Cherrington, Alan D. .

JOURNAL OF CLINICAL INVESTIGATION, 2012, 122 (01) :4-12

[6]

Association of fasting glucagon and proinsulin concentrations with insulin resistance [J].

Ferrannini, E. ;

Muscelli, E. ;

Natali, A. ;

Gabriel, R. ;

Mitrakou, A. ;

Flyvbjerg, A. ;

Golay, A. ;

Hojlund, K. .

DIABETOLOGIA, 2007, 50 (11) :2342-2347

[7]

Differentiation phenotypes of pancreatic islet β- and α-cells are closely related with homeotic genes and a group of differentially expressed genes.[J].Noriko Mizusawa;Tomoko Hasegawa;Izumi Ohigashi;Chisato Tanaka-Kosugi;Nagakatsu Harada;Mitsuo Itakura;Katsuhiko Yoshimoto.Gene.2004,

[8]

Hypoglycemic action of Cyclocarya paliurus (Batal.) Iljinskaja in normal and diabetic mice [J].

Kurihara, H ;

Fukami, H ;

Kusumoto, A ;

Toyoda, Y ;

Shibata, H ;

Matsui, Y ;

Asami, S ;

Tanaka, T .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2003, 67 (04) :877-880

[9]

Insulin inhibits glucagon secretion by the activation of PI3-kinase in In-R1-G9 cells.[J].K. Kaneko;T. Shirotani;E. Araki;K. Matsumoto;T. Taguchi;H. Motoshima;K. Yoshizato;H. Kishikawa;M. Shichiri.Diabetes Research and Clinical Practice.1999, 2

[10]

EXPRESSION OF INSULIN-RECEPTOR ON CLONAL PANCREATIC ALPHA-CELLS AND ITS POSSIBLE ROLE FOR INSULIN-STIMULATED NEGATIVE REGULATION OF GLUCAGON-SECRETION [J].

KISANUKI, K ;

KISHIKAWA, H ;

ARAKI, E ;

SHIROTANI, T ;

UEHARA, M ;

ISAMI, S ;

URA, S ;

JINNOUCHI, H ;

MIYAMURA, N ;

SHICHIRI, M .

DIABETOLOGIA, 1995, 38 (04) :422-429

← 1 2 →