强直性脊柱炎和类风湿关节炎患者外周血单个核细胞中Toll样受体4基因的表达及临床意义

被引:4
作者
杨再兴
梁艳
郝婉莹
林志跃
赵文静
仲人前
机构
[1] 第二军医大学附属长征医院实验诊断科
关键词
Toll样受体4; 强直性脊柱炎; 类风湿关节炎; 实时RT-PCR; HLA-B27;
D O I
暂无
中图分类号
R593 [免疫性疾病];
学科分类号
摘要
目的探讨Toll样受体4(TLR4)mRNA在强直性脊柱炎(AS)、类风湿关节炎(RA)患者以及健康个体外周血单个核细胞(PBMC)中的表达差异及其临床意义。方法应用实时荧光定量逆转录聚合酶链反应(RT-PCR)检测2006年3月至10月第二军医大学附属长征医院60例AS患者、20例RA患者及30例健康对照PBMC中TLR4 mRNA的表达水平;应用ESR自动化分析仪和特定蛋白电泳仪分别测定红细胞沉降率(ESR)和血浆C-反应蛋白(CRP)。结果AS和RA患者PBMCTLR4 mRNA的表达水平明显高于对照组,但两疾病组之间差异并无显著性意义;人类白细胞抗原(HLA-B27)阳性和阴性AS患者均显著高于健康对照组,且HLA-B27阳性AS患者显著高于RA组,但两组AS患者以及HLA-B27阴性AS与RA患者之间,TLR4 mRNA差异无显著性意义;在HLA-B27阳性AS患者中,TLR4 mRNA的表达与ESR及CRP呈显著相关性,但在HLA-B27阴性AS患者中则无这种相关性。在RA患者中,TLR4 mRNA与CRP呈显著相关性。结论AS和RA患者PBMCTLR4 mRNA的表达均上调,AS患者上调与HLA-B27阳性与否无关,但HLA-B27阳性AS患者比RA上调更明显,且HLA-B27抗原影响AS患者TLR4 mRNA与ESR及CRP的相关性。TLR4的异常表达可能在AS和RA的发生和发展中起作用。
引用
收藏
页码:938 / 941
页数:4
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