钙调蛋白的制备及活性测定

被引：61

作者：

刘景生

刘晔

金荫昌

机构：

[1] 中国医学科学院基础所药理室,中国医学科学院基础所药理室,中国医学科学院基础所药理室北京,北京,北京

来源：

中国医学科学院学报 | 1985年 / 06期

关键词：

钙调蛋白; Ca; 活性测定; PDE;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

本文介绍了一个高度敏感的CaM活性测定法,该测定法以在Ca存在下CaM能刺激磷酸二酯酶的活性为基础。磷酸二酯酶从牛心肌提取,经过DEAE-cellulose柱层析纯化,用Lowry法测定蛋白含量为5mg/ml,2μl相当于1个活性单位。CaM从大鼠睾丸匀浆液中提取,经Fluphenazine-Sepharose亲和层析柱分离提纯。纯化CaM在SDS-凝胶电泳上显示为一个单一的蛋白带,分子量为16～17K道尔顿,0.5ng为一个活性单位。本文介绍的CaM活性测定法,敏感度为0.1ng,方法简便、可靠,重复性好。

引用

页码：453 / 458

页数：6

共 14 条

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