实时荧光PCR检测志贺菌特异基因ipaH

被引：2

作者：

柳健 ^{[1
]}

石建凤 ^{[2
]}

丁昌平 ^{[1
]}

张敏 ^{[1
]}

曹春梅 ^{[1
]}

机构：

[1] 江苏省扬州市第一人民医院中心实验室

[2] 山东省烟台市传染病医院检验科

来源：

中国卫生检验杂志 | 2007年 / 11期

关键词：

志贺菌; 实时荧光PCR; ipaH基因;

D O I：

暂无

中图分类号：

R450 [];

学科分类号：

100215 ;

摘要：

目的:建立一种灵敏、快速检测志贺菌的实时荧光PCR方法,研究志贺菌在腹泻病人中的感染情况。方法:针对志贺菌特异性基因ipaH设计引物、探针,应用培养分离的志贺菌进行验证,同时检测360例临床标本并与培养法进行对照。结果:应用实时荧光PCR方法,10株志贺菌检测结果均为阳性,10株非志贺菌结果均为阴性;检测灵敏度达到4 cfu/test。360例临床标本中实时荧光PCR方法检测出29例阳性,培养法检测出12例阳性。结论:实时荧光PCR法检测志贺菌具有灵敏度高、快速方便的特点,可用于检测腹泻病人志贺菌感染。

引用

页码：2041 / 2042

页数：2

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