IL-15基因真核表达质粒的构建及其对CVB3 VP1基因疫苗的免疫增强作用

被引:4
作者
房文亮
王永祥
金玉怀
金士香
周雪燕
机构
[1] 河北医科大学基础医学院病原生物学教研室
[2] 河北医科大学基础医学院病原生物学教研室 河北石家庄
[3] 河北石家庄
关键词
白细胞介素-15; 质粒; 柯萨奇病毒B组; 小鼠;
D O I
暂无
中图分类号
R392.1 [免疫生物学];
学科分类号
100102 ;
摘要
目的探讨人白细胞介素 15 (humaninterleukin 15 ,hIL 15 )基因佐剂对柯萨奇B3病毒 (CoxsackievirusB3 ,CVB3 )VP1基因疫苗的免疫增强效果。方法从人胚肺二倍体细胞提取hIL 15的mRNA ,用逆转录 聚合酶链反应 (reversetranscriptase polymerasechainreaction ,RT PCR)扩增出cDNA ,构建成真核表达克隆 pcDNA3 IL 15。将pcDNA3 VP1、pcDNA3 VP1+ pcDNA3 IL 15、pcDNA3 IL 15、pcDNA3和盐水分别肌内注射Balb/c小鼠 ,每次免疫后 6天取血清用微量中和试验测CVB3中和抗体 ,3次免疫后用 80 0TCID5 0CVB3感染小鼠 ,观察小鼠的生存率。结果 pcDNA3 IL 15重组质粒的目的基因与hIL 15基因序列相同 ;pcDNA3 VP1和pcDNA3 VP1+ pcDNA3 IL 15能诱导小鼠产生中和抗体 ,抗体滴度随免疫次数增加而提高 ;pcDNA3 VP1+ pcDNA3 IL 15组抗体滴度和生存率均高于 pcDNA3 VP1组。结论本研究成功构建了hIL 15重组质粒 ,该质粒对真核表达重组质粒 pcDNA3 VP1有一定的免疫原性增强作用
引用
收藏
页码:129 / 132
页数:4
相关论文
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