原花青素减轻TCP磨损颗粒诱导的骨细胞氧化损伤

被引：3

作者：

金晶晶

楼佳琪

王脉

陈晓斌

杨子键

黄君瑶

张云

机构：

[1] 绍兴文理学院医学院

来源：

中国病理生理杂志 | 2019年 / 35卷 / 09期

基金：

浙江省自然科学基金;

关键词：

磷酸三钙磨损颗粒; 骨细胞; 原花青素; 氧化应激; NLRP3炎症小体; 细胞焦亡;

D O I：

暂无

中图分类号：

R684 [关节疾病及损伤];

学科分类号：

100220 [骨科学];

摘要：

目的:研究原花青素(PC)对磷酸三钙(TCP)磨损颗粒诱导的骨细胞氧化损伤的影响,并探讨其可能的作用机制。方法:将TCP磨损颗粒(0.1 g/L)与小鼠长骨MLO-Y4骨细胞共孵育构建骨细胞体外损伤模型,实验分为4组:正常对照(control)组、TCP组、PC(10μmol/L)组和PC(50μmol/L)组。应用Calcein-AM染色和MTT等方法检测骨细胞活力;ELISA检测细胞培养上清液中牙本质基质蛋白1(DMP-1)、骨硬化蛋白(SOST)及白细胞介素1β(IL-1β)水平;流式细胞术定量分析骨细胞凋亡情况;化学比色法检测骨细胞中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,以及细胞培养上清液中乳酸脱氢酶(LDH)释放量;Western blot法检测骨细胞中NOD样受体蛋白3(NLRP3)、含CARD的凋亡相关斑点样蛋白(ASC)、cleaved caspase-1和IL-1β蛋白水平的变化。结果:与control组比较,TCP组MLO-Y4细胞的损伤、凋亡率及MDA含量显著增加(P<0.05),SOD活性显著降低(P<0.05),NLRP3、ASC、cleaved caspase-1和IL-1β蛋白水平显著上调,上清液中IL-1β和LDH的水平明显增加(P<0.05);与TCP组比较,PC组MLO-Y4细胞的损伤明显减轻,凋亡率显著降低(P<0.05),NLRP3、ASC、cleaved caspase-1和IL-1β的蛋白水平显著下调(P<0.05),IL-1β和LDH水平明显降低(P<0.05)。结论:PC可明显抑制TCP磨损颗粒诱导的骨细胞氧化损伤,其机制可能与减轻NLRP3炎症小体的活化和细胞焦亡相关。

引用

页码：1676 / 1682

页数：7

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