A multiple stable isotope tracer technique forStudying the metabolic kinetics of amino acids in hepatic failure

被引：1

作者：

Xia Zongqin Dai Tengchang Zhang Jianhua Hu Yaer Yu Bingyao Xu Xingrong Huang Guanlu(Shanghai Second Medical University)Shen Gengrong Zhou Yaqiu Yu Hong Lu Hanming Li Xunhai(Shanghai Ruijin Hospital) (Shanghai Xinhau Hospital)

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来源：

核技术 | 1987年 / 08期

关键词：

gas chromatography-mass spectrometry amino acids hepatic failure stable isotope tracer;

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摘要：

<正> In order to study the mechanism of the imbalance of amino acid metabolism during hepatic failure, a stable isotope tracer method for observing simultaneously the metabolic kinetics of several amino acids has been established. 15N-L-Ala, (2, 3-D3)-Leu and (2, 3-D3) - Phe were chosen as nonessential, branched chain and aromatic amino acids. A. single iv injection of 40 mg 15N-Ala, 20 mg deuterated Leu and 20 mg deuterated Phe was given to each human subject. Blood samples were taken just before and at different times (up to 60 rain) after the injection. Total free amino acids were isolated from the plasma with a small dowex 50 x 8 column and converted to trifluoroacetyl derivatives. Their abundances were then analyzed with a GC-MS system and typical double exponential time course curves were found for all the three labelled amino acids. A two-pool model was designed and applied for compartmental analysis.Significant changes were found in the kinetic parameters of Phe and Leu in patients with fulminant hep

引用

页码：45 / 53

页数：9