病理状态对心肌细胞及心脏成纤维细胞β3-肾上腺素受体表达的影响

被引：3

作者：

徐忠诚 ^{[1
]}

辛俊舟 ^{[1
]}

吴磊 ^{[1
]}

刘红阳 ^{[2
]}

王丽 ^{[1
]}

机构：

[1] 石河子大学医学院第一附属医院心内三科

[2] 石河子大学医学院第一附属医院老干一科

来源：

天津医药 | 2019年 / 47卷 / 07期

关键词：

肌细胞,心脏; 成纤维细胞; 去甲肾上腺素; β3-肾上腺素受体; 血管紧张素Ⅱ;

D O I：

暂无

中图分类号：

R54 [心脏、血管（循环系）疾病];

学科分类号：

100201 [内科学];

摘要：

目的观察与心力衰竭相关的不同病理因素刺激下,Sprague-Dawley(SD)乳鼠心肌细胞和心脏成纤维细胞中β3-肾上腺素受体(β3-AR)表达变化的差异。方法分离培养SD乳鼠心肌细胞和心脏成纤维细胞并行免疫荧光鉴定,分别给予血管紧张素Ⅱ(AngⅡ)及去甲肾上腺素(NE)进行刺激,采用实时荧光定量PCR(qPCR)检测心肌细胞肥大相关基因[心钠肽(ANP)、脑钠肽(BNP)、β-肌球蛋白重链(β-MHC)]、心脏成纤维细胞纤维化相关基因[Ⅰ型胶原蛋白(COL-Ⅰ)、Ⅲ型胶原蛋白(COL-Ⅲ)]及2种细胞中β3-AR的mRNA表达。结果免疫荧光鉴定显示,分离培养的心肌细胞和心脏成纤维细胞均状态良好。AngⅡ和NE分别刺激2种细胞48 h后,心肌细胞中ANP、BNP、β-MHC和β3-AR的表达较对照组明显增加;心脏成纤维细胞中COL-Ⅰ、COL-Ⅲ表达较对照组明显增加,但β3-AR的表达没有明显变化。结论参与心力衰竭发生发展的病理因素AngⅡ和NE主要引起心肌细胞的β3-AR表达增加,而不是心脏成纤维细胞。

引用

页码：700 / 704

页数：5

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